cc_byHiller, EricHermann, AlexanderLilge, Lars2026-01-302026-01-302025https://doi.org/10.1007/s12268-025-2564-zhttps://hohpublica.uni-hohenheim.de/handle/123456789/18309Bioproduction of bioactive compounds on a large-scale poses challenges that require molecular strain engineering. By integrating the principle of genetic code expansion (GCE), the biosynthesis of the surfactin biosurfactant has been controlled in the production host Bacillus subtilis depending on the supply of a defined non-canonical amino acid. The regulation of this molecular process control has been transferred to high-cell-density fed-batch bioreactor procedures, allowing an initial upscaling.gerGenetic code expansionNon-canonical amino acidsBacillus subtilisSurfactin660Article2025-11-04