Newest publications
Smoking is associated with increased eryptosis, suicidal erythrocyte death, in a large population-based cohort
(2024) Schmitt, Marvin; Ewendt, Franz; Kluttig, Alexander; Mikolajczyk, Rafael; Kraus, F. Bernhard; Wätjen, Wim; Bürkner, Paul-Christian; Stangl, Gabriele I.; Föller, Michael
Smoking has multiple detrimental effects on health, and is a major preventable cause of premature death and chronic disease. Despite the well-described effect of inhaled substances from tobacco smoke on cell toxicity, the association between smoking and suicidal erythrocyte death, termed eryptosis, is virtually unknown. Therefore, the blood samples of 2023 participants of the German National Cohort Study (NAKO) were analyzed using flow cytometry analysis to determine eryptosis from fluorescent annexin V-FITC-binding to phosphatidylserine-exposing erythrocytes. Blood analyses were complemented by the measurement of hematologic parameters including red blood cell count, hematocrit, hemoglobin, mean corpuscular cell volume (MCV) and mean corpuscular hemoglobin (MCH). Eryptosis was higher in smokers than in non- and ex-smokers, and positively associated with the number of cigarettes smoked daily ( r = 0.08, 95% CI [0.03, 0.12]). Interestingly, despite increased eryptosis, smokers had higher red blood cell indices than non-smokers. To conclude, smokers were characterized by higher eryptosis than non-smokers, without showing any obvious detrimental effect on classic hematological parameters.
Simulation of dual-purpose chicken breeding programs implementing gene editing
(2024) Chuang, Edward Y. S.; Wellmann, Robin; Meijboom, Franck L. B.; Tetens, Jens; Bennewitz, Jörn
Background: In spite of being controversial and raising ethical concerns, the application of gene editing is more likely to be accepted when it contributes to improving animal welfare. One of the animal welfare and ethical issues in chicken breeding is chick culling, the killing of the male layer chicks after hatching due to the poor fattening performance. Although establishing dual-purpose chicken lines could solve this problem, unfavorable genetic correlations between egg and meat production traits hindered their competitiveness. Although it is also controversial in ethical terms, gene editing may accelerate genetic progress in dual-purpose chicken and alleviate the ethical concerns from chick culling.
Results: The simulation compared the utility improvement in dual-purpose use under two breeding schemes: one consisting in the improvement of the laying hens, and the second in the improvement of a synthetic line obtained from a layer broiler cross. In each breeding scheme, the breeding programs were simulated with and without gene editing. Polygenic breeding values and 500 simulated quantitative trait loci (QTL) with different levels of pleiotropy caused negative correlations between egg production, meat production, and overall health. The results of the simulation demonstrated that genetic gain could be accelerated by at most 81% for several generations if gene editing was used. The actual increase in genetic gain depended on the number of single nucleotide polymorphisms (SNPs) being edited per animal. The rate of genetic improvement became equal in scenarios with and without gene editing after 20 generations. This is because the remaining segregating QTL had small effects and their edition would have negative overall health effects from potential off-target edits. Although gene editing can improve genetic gain in quantitative traits, it can only be recommended as long as QTL with reasonable effect sizes are segregating and detectable.
Conclusions: This simulation demonstrates the potential of gene editing to accelerate the simultaneous improvement of negatively correlated traits. When the risk of negative consequences from gene editing persists, the number of SNPs to be edited should be chosen carefully to obtain the optimal genetic gain.
Evaluation of energetic potential of slaughterhouse waste and its press water obtained by pressure-induced separation via anaerobic digestion
(2024) Yankyera Kusi, Joseph; Empl, Florian; Müller, Ralf; Pelz, Stefan; Poetsch, Jens; Sailer, Gregor; Kirchhof, Rainer; Agyemang Derkyi, Nana Sarfo; Attiogbe, Francis; Siabi, Sarah Elikplim; Jeon, Byong-Hun
Anaerobic digestion has the potential to convert organic waste materials into valuable energy. At the same time, using press water from biomass materials for energy generation while taking advantage of the resulting cake for other purposes is an emerging approach. Therefore, this study aimed to investigate the residual potential expected from a typical biogas feedstock after it has been mechanically separated into liquid and solid phases. Hence, in this study, the rumen contents of ruminants (cow, goat, and sheep) and their proportionate ratios were obtained from an abattoir in Ghana. Resource characterization of the waste samples was carried out in the central laboratory of the HFR, Germany. Anaerobic batch tests for biogas (biomethane) yield determination were set up using the Hohenheim Biogas Yield Test (HBT). The inoculum used was obtained from an inoculum production unit at the Hohenheim University biogas laboratory. The trial involved two different forms of the sample: mixture of rumen contents, press water, and inoculum, each in four (4) replicates. The trial was carried out at a mesophilic temperature of 37 °C. Results obtained over a seventy (70) day period were transformed into biogas yields. Overall, the results show that the current contents are suitable for biogas generation as an option as opposed to the current form of disposal at a refuse dump. However, using these mixtures in their original forms is more technically viable than using press water without further treatment.
Effect of irrigation canal conveyance efficiency enhancement on crop productivity under climate change in Nepal
(2024) Basukala, Amit Kumar; Eschenbach, Annette; Rasche, Livia
Nepal is expanding its irrigation facilities as an adaptive measure to climate change; however, the current canal conveyance efficiency (CCE) is low with significant water losses. In this study, we assess the potential impact of increasing CCE on the productivity of rice, maize, and wheat under different climate change scenarios (SSP1-2.6, SSP3-7.0, and SSP5-8.5), utilizing three bias-adjusted general circulation models. The study simulates potential yields at ecoregion levels for two periods: near future (2023 to 2050) and end-century (2075 to 2100). Management scenarios include the following: (1) business as usual, (2) CCE at 30%, (3) CCE at 50%, and (4) CCE at 70%. The results indicate that increasing CCE to 30%, coupled with expanded irrigated areas and adjusted fertilization rates, could boost yields by three tons per hectare across all three crops at the national level. Further increasing CCE to 50% could yield additional increases of up to 0.6 t/ha of maize and 1.2 t/ha of rice in the terai region. A CCE of 70% results in further increases of up to 2.1 t/ha of rice and 1.2 t/ha of maize. The benefits of improved CCE vary by location, with the subtropical terai region experiencing the most and the mountain regions showing the least. We conclude that there is potential to increase yields by increasing CCE to 70% in the terai region, 50% in the hill region, and 30% in the mountains. Wheat appears to benefit the least from improved CCE. This work highlights efficient irrigation as a reliable adaptive measure for future climate change in Nepal.
Interactions between lipid oxidation and anthocyanins from black carrots in ω-3 fatty acid-rich flaxseed oil-in-water emulsions
(2024) Klinger, Evelyn; Salminen, Hanna; Bause, Karola; Weiss, Jochen
The application of anthocyanins as red colorants in lipid-containing foods such as oil-in-water emulsions is challenging due to their ability to act as antioxidants and their instability under various environmental conditions. In this study, we investigated the kinetics of black carrot anthocyanin degradation and the subsequent color loss influenced by lipid oxidation reactions in 1% ( w/w ) flaxseed oil-in-water emulsions stabilized by 0.1% ( w/w ) sodium dodecyl sulfate (SDS) at pH 2 upon storage at 35 °C for ten days under light and in the dark. Oxidatively stable Miglyol oil-in-water emulsions and SDS solutions were used as reference. The results showed simultaneous formation of lipid hydroperoxides and degradation of anthocyanins. The addition of anthocyanins decreased lipid hydroperoxide formation, confirming the antioxidant activity of anthocyanins through radical scavenging mechanism. The kinetic modelling of lipid oxidation and anthocyanin degradation are particularly important for estimating color stability in colored emulsion-based food systems such as dairy or non-dairy milk or yoghurt drinks.