Browsing by Person "Hahn, Volker"
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Publication Do we need to breed for regional adaptation in soybean? - Evaluation of genotype-by-location interaction and trait stability of soybean in Germany(2023) Döttinger, Cleo A.; Hahn, Volker; Leiser, Willmar L.; Würschum, TobiasSoybean is a crop in high demand, in particular as a crucial source of plant protein. As a short-day plant, soybean is sensitive to the latitude of the growing site. Consequently, varieties that are well adapted to higher latitudes are required to expand the cultivation. In this study, we employed 50 soybean genotypes to perform a multi-location trial at seven locations across Germany in 2021. Two environmental target regions were determined following the latitude of the locations. Adaptation and trait stability of seed yield and protein content across all locations were evaluated using Genotype plus Genotype-by-Environment (GGE) biplots and Shukla’s stability variance. We found a moderate level of crossing-over type genotype-by-location interaction across all locations. Within the environmental target regions, the genotype-by-location interaction could be minimised. Despite the positive correlation (R = 0.59) of seed yield between the environmental target regions and the same best-performing genotype, the genotype rankings differed in part substantially. In conclusion, we found that soybean can be grown at a wide range of latitudes across Germany. However, the performance of genotypes differed between the northern and southern locations, with an 18.8% higher mean yield in the south. This in combination with the observed rank changes of high-performing genotypes between both environmental target regions suggests that selection targeted towards environments in northern Germany could improve soybean breeding for those higher latitude regions.Publication Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling(2023) Rozhon, Wilfried; Ramirez, Veronica E.; Wieckhorst, Silke; Hahn, Volker; Poppenberger, BrigitteSunflower (Helianthus annuus L.) is the second most important oil seed crop in Europe. The seeds are used as confection seeds and, more importantly, to generate an edible vegetable oil, which in normal varieties is rich in the polyunsaturated fatty acid linoleic acid. Linoleic acid is biosynthesized from oleic acid through activity of the oleate desaturase FATTY ACID DESATURASE 2 (FAD2), which in seeds is encoded by FAD2-1, a gene that’s present in single copy in sunflowers. Defective FAD2-1 expression enriches oleic acid, yielding the high oleic (HO) acid trait, which is of great interest in oil seed crops, since HO oil bears benefits for both food and non-food applications. Chemical mutagenesis has previously been used to generate sunflower mutants with reduced FAD2-1 expression and here it was aimed to produce further genetic material in which FAD2-1 activity is lost and the HO trait is stably expressed. For this purpose, a sunflower mutant population was created using gamma irradiation and screened for fad2-1 mutants with a newly developed HPLC-based fatty-acid profiling system that’s suitable for high-throughput analyses. With this approach fad2-1 knock-out mutants could be isolated, which stably hyper-accumulate oleic acid in concentrations of 85-90% of the total fatty acid pool. The genetic nature of these new sunflower lines was characterized and will facilitate marker development, for the rapid introgression of the trait into elite sunflower breeding material.Publication How can we breed for phosphate efficiency in maize (Zea mays)?(2022) Weiß, Thea M.; Li, Dongdong; Roller, Sandra; Liu, Wenxin; Hahn, Volker; Leiser, Willmar L.; Würschum, TobiasFuture farming is required to produce high yields with reduced inputs. Increased fertilizer prices and policy goals underline the need to breed for nutrient‐efficient varieties. We therefore conducted a multienvironmental field trial comprising 400 maize genotypes, half elite lines and half doubled haploid lines from six European landraces and assessed yield parameters and corresponding phosphorus concentrations at two developmental stages. From these traits, we derived several measures for phosphate efficiency and evaluated them phenotypically and genetically. The results of this study revealed that ample variation for phosphate efficiency is present in maize. However, while elite material clearly outperformed all landraces with regard to yield‐related traits, some landrace genotypes indicated superior early development characteristics. The phosphate efficiency measures showed a complex genetic architecture, and hence, genomic selection appears best suited to assist their improvement. Taken together, breeding for phosphate efficiency is feasible but should be performed under the same conditions in which the crops are eventually grown because phosphate efficiency and what is deemed a sustainable P balance largely depends on the context.