Institut für Kulturpflanzenwissenschaften

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Assessment of different methods to determine NH₃ emissions from small field plots after fertilization
(2025) Götze, Hannah; Brokötter, Julian; Frößl, Jonas; Kelsch, Alexander; Kukowski, Sina; Pacholski, Andreas Siegfried; Anderson, William A.
Ammonia (NH₃) emissions affect the environment, climate and human health and originate mainly from agricultural sources like synthetic nitrogen fertilizers. Accurate and replicable measurements of NH₃ emissions are crucial for research, inventories and evaluation of mitigation measures. There exist specific application limitations of NH₃ emission measurement techniques and a high variability in method performance between studies, in particular from small plots. Therefore, the aim of this study was the assessment of measurement methods for ammonia emissions from replicated small plots. Methods were evaluated in 18 trials on six sites in Germany (2021–2022). Urea was applied to winter wheat as an emission source. Two small-plot methods were employed: inverse dispersion modelling (IDM) with atmospheric concentrations obtained from Alpha samplers and the dynamic chamber Dräger tube method (DTM). Cumulative NH₃ losses assessed by each method were compared to the results of the integrated horizontal flux (IHF) method using Alpha samplers (Alpha IHF) as a micrometeorological reference method applied in parallel large-plot trials. For validation, Alpha IHF was also compared to IHF/ZINST with Leuning passive samplers. Cumulative NH₃ emissions assessed using Alpha IHF and DTM showed good agreement, with a relative root mean square error (rRMSE) of 11%. Cumulative emissions assessed by Leuning IHF/ZINST deviated from Alpha IHF, with an rRMSE of 21%. For low-wind-speed and high-temperature conditions, NH3 losses detected with Alpha IDM had to be corrected to give acceptable agreement (rRMSE 20%, MBE +2 kg N ha−1). The study shows that quantification of NH₃ emissions from small plots is feasible. Since DTM is constrained to specific conditions, we recommend Alpha IDM, but the approach needs further development.
Improving cover crop mixtures to increase soil carbon inputs and weed suppression as a tool to promote yield potential
(2024) Groß, Jonas; Müller, Torsten
Arable cropping systems are facing challenges imposed by climate change and are, at the same time, a tool to mitigate climate change. Soils are essential in securing yield potential and acting as a carbon sink. Recognizing small-scale site-specific differences in crop management and integrating cover crops, which provide ecosystem services such as carbon sequestration and weed suppression, are two approaches to climate-smart agriculture. To investigate site-specific soil heterogeneity, soil properties were analyzed in a field trial, measuring at three soil depths in 42 plots to determine their influence on yield measures. Soil organic carbon, silt, and clay contents in both topsoil and subsoil explained 45-46% of the variability in grain yield. Additionally, a positive correlation was found between increasing clay content in the topsoil and grain yield and tiller density. A higher clay content in the subsoil resulted in a decrease in grain yield. Soil organic carbon was identified as a soil property that positively influences yield and yield formation at any soil depth through multiple regressions and cluster analysis. Soil organic carbon is a critical soil measure that can significantly improve yield potential and can be manipulated by crop management practices like cover cropping. In a second field experiment, the impacts of increasing plant diversity of cover crop mixtures on rhizosphere carbon input and microbial utilization were investigated. A comparison was made between Mustard (Sinapis alba L.) planted as a sole crop and diversified cover crop mixtures of four (Mix4) or twelve (Mix12) species. A 13C-pulse labeling field experiment traced C transfer from shoots to roots to the soil microbial community. Mix 4 doubled the net CO2-C removal from the atmosphere, while Mix 12 more than tripled it, indicating that plant diversity positively impacts carbon cycling. This is reflected in higher atmospheric C uptake, higher transport rates to the rhizosphere, higher microbial incorporation, and longer residence time in the soil environment, improving the efficiency of C cycling in cropping systems. Root C-transfer could be identified as a fast pathway for C to reach soil C-compartments, but a substantial share of atmospheric C-catch comes from shoot biomass. In a third field experiment, the influence of species combination on shoot biomass formation was systematically assessed by investigating species interactions in dual cover crop mixtures and their competitiveness to suppress weeds before winter under different growing conditions. The shoot biomass share of a cover crop species in a dual-species mixture was found to be directly linked to its shoot biomass in a pure stand. Mustard and phacelia had similar effects on the shoot biomass production of the second species added to the mixture. Cruciferous species were more competitive against weeds than other cover crop species and could suppress weeds even when mixed with a less competitive partner. Weed suppression in mixtures with phacelia depended on the second component. Our results indicate that dual mixtures containing one competitive species reduce weed shoot biomass before winter, comparable to competitive pure stands. The research in this thesis shows that C content in the soil plays a crucial role in yield formation in arable cropping systems in Germany. Finally, the study has demonstrated that implementing cover crop mixtures can enhance soil C input and represent a valuable method for preserving yield potential. It was also shown that an intelligent combination of cover crop species can determine successful development and weed suppression.
Testing agronomic treatments to improve the establishment of novel miscanthus hybrids on marginal land
(2025) Lewin, Eva; Clifton‐Brown, John; Jensen, Elaine; Lewandowski, Iris; Krzyżak, Jacek; Pogrzeba, Marta; Hartung, Jens; Wolfmüller, Cedric; Kiesel, Andreas; Fujii, Yoshiharu
Miscanthus is considered a promising candidate for the cultivation of marginal land. This land poses unique challenges, and experiments have shown that the “establishment phase” is of paramount importance to the long-term yield performance of miscanthus. This experiment analyzes novel miscanthus hybrids and how their establishment on marginal land can be improved through agronomic interventions. Experiments took place at two sites in Germany: at Ihinger Hof, with a very shallow soil profile and high stone content, and at Reichwalde, where the soil was repurposed river sediment with low organic matter, high stone content, and a compacted lower horizon. These marginal conditions functioned as test cases for the improvement of miscanthus establishment agronomy. Four hybrids ( Miscanthus x giganteus , Gnt10, Gnt43, and Syn55) and agronomic treatments such as plastic mulch film, miscanthus mulch, inoculation with mycorrhizal fungi, and fertilization were tested in two years at both sites in 2021 and 2022. Specific weather conditions and the timing of planting were strong determinants of establishment success and no single treatment combination was found that consistently increased the establishment success. Plastic mulch films were found to hinder rather than help establishment in both these locations. Chipped miscanthus mulch caused nitrogen immobilization and stunted plant growth. At Ihinger Hof the novel seed-based miscanthus hybrid Gnt43 produced twice the biomass of other hybrids (7 t ha −1 ) in the first growing season. Gnt10 yielded well in 2021 and showed impressive tolerance to water stress in the summer of 2022. No treatment combination was found that consistently increased the establishment success of miscanthus hybrids across sites and years. Novel genotypes consistently outperformed the standard commercial miscanthus hybrid Miscanthus x giganteus . Gnt10 may be a promising candidate for the cultivation of water-stress-prone marginal lands, due to its isohydric behavior and high yield potential.
Yield stability and weed dry matter in response to field-scale soil variability in pea-oat intercropping
(2025) Munz, Sebastian; Zachmann, Julian; Chongtham, Iman Raj; Dhamala, Nawa Raj; Hartung, Jens; Jensen, Erik Steen; Carlsson, Georg
Background and aims: Intercropping of grain legumes and cereals in European agriculture can provide benefits, such as an increase in yields, yield stability and weed suppression. Interactions between crops in intercropping may depend on spatial heterogeneity in soil conditions, which are present on farmers’ fields. Understanding the effect of within-field variation in soil conditions on interspecific interactions might increase the benefits of intercropping by within-field adjustment of the agronomic management. Methods: Crop performance and weed dry matter were assessed together with several soil properties in grids within three large field experiments at two sites (Germany and Sweden) and during two years. Each experiment was comprised of several strips sown either with the two sole crops oat ( Avena sativa L.) and field pea ( Pisum sativum L.) or an oat-pea intercrop. Results: The response of crop performance to within-field variability in soil conditions was mostly species-specific. Yield stability of intercropping was consistently higher compared with pea, but not compared to oat. The highest land equivalent ratio was found for an additive intercropping design under a higher water availability. In this experiment, yield stability of both intercropped pea and oat were lower, which might be expected as a result of within-field variation in interspecific interactions. Intercropping reduced weed dry matter compared to pea, for which one experiment indicated an increase in weed dry matter with nutrient availability. Conclusion: The experimental design and the developed statistical analysis can contribute to further research about spatial variations in interspecific interactions in intercropping, which will improve the understanding of plant-plant and plant-soil interactions.

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