Browsing by Subject "Batate"

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Physiological mechanisms and growth responses of sweet potato subjected to salinity
(2023) Mondal, Shimul; Asch, Folkard
For the development of salt-tolerant sweet potato varieties, either through breeding or biotechnology, an appropriate salinity screening tool is necessary for the identification of tolerant or sensitive genotype. Our overall objectives for this study were to develop a suitable, reliable and rapid salinity screening tool in view of salt tolerance mechanism in sweet potato under salinity. To better understand the tolerance mechanisms; leaf level ion uptake and distribution patterns by transpirational water loss and leaf level ROS scavenging antioxidant enzyme activities were evaluated under salinity. Additionally, different ion extraction methods were tested which will contribute to the development of reliable salinity screening tool in sweet potato genotypes. All the experiments were conducted in the greenhouse and VPD (vapor pressure deficit) chambers of the Hans-Rutenberg Institute of Tropical Agricultural Sciences, University of Hohenheim, Germany, in a hydroponic system. Twelve genotypes of sweet potato were collected from Bangladesh Agricultural Research Institute (BARI) and used to evaluate salt thresholds with salt tolerance mechanisms for a wide range of salinity levels (0, 50, 100, and 150 mM NaCl). First, genotypic thresholds were determined for 12 sweet potato genotypes exposed to salinity, whereupon it was found that 75 mM root zone salinity (NaCl) was the threshold for sweet potato. The genotypic threshold was estimated from the dry matter accumulation that began to decrease under the influence of salinity. It was found that genotypic thresholds were negatively linearly correlated with the difference between tissue K content at 75 mM NaCl and tissue K content at controlled salinity in the root zone. This information is very important for identifying the salt tolerant and sensitive genotype of sweet potato. Second, the uptake and distribution of Na, K, and Cl ions by transpiration, across different-aged leaves, were studied to better understand the mechanisms of salt tolerance in sweet potato. Two different sweet potato genotypes were subjected to salt stress of 0 and 50 mM NaCl in artificially dry (VPD 2.27 kPa) and humid (VPD 0.76 kPa) chambers. We found that cumulative water loss per unit leaf area was twice as high at a VPD of 2.27 kPa, but Na uptake remained the same. No relationship was observed between water loss from individual leaves and Na or Cl uptake. About 30% more Na was distributed in the petioles of salt tolerant genotype compared to leaf blades, while the opposite was observed in salt sensitive sweet potato genotype and VPD had no effect on Na distribution. Third, the activities of ROS scavenging antioxidant enzymes were evaluated with respect to different leaf age, in two different genotypes of sweet potato under 100 mM salinity. In general, antioxidant enzymes in sweet potato do not respond to salt stress but are altered by the effects of leaf position, leaf age, duration of stress, and genotype. No effect of Na on antioxidant enzyme activities was found under salt stress in sweet potato leaves. However, the significant positive correlation between K concentration and the level of SOD (super oxide dismutase) in older leaves suggests that SOD contributes to the maintenance of a high K concentration to protect photosynthetic activity. In summary, this study shows that sweet potato responds differently to salinity depending on the genotype, and that the threshold beyond which yield decreases is 75 mM NaCl. Genotypic threshold strongly linked to high tissue K content under increasing salinity that suggests a salt tolerance mechanisms in sweet potato. Salt-tolerant sweet potatoes distribute significant amounts of Na and K in their petioles. Young leaves of the tolerant genotype contain more K under salt stress. GR and positive relationship between K concentration and SOD in salt tolerant genotypes indicate some tolerance mechanisms. So, a screening tool is proposed for sweet potato based on the genotypic ability to maintain high tissue K levels under increasing salinity level.