Browsing by Subject "Carbon use efficiency"

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Constraints on microbial pesticide degradation in soils
(2023) Wirsching, Johannes; Kandeler, Ellen
Pesticides are an essential component of intensified agriculture and have contributed significantly to the increase in food production observed in recent decades. Since 1960, pesticide use has increased by a factor of fifteen to twenty, representing a market value of $40 billion in 2016. Soil monitoring campaigns to track pesticide contamination of croplands across Europe are quantifying pesticide residues whose residence times in soils exceed expected values. Diffuse contamination by pesticide residues raises concerns about soil functions, soil biodiversity, and food safety, as well as the transport of contaminants by wind and water to surface waters or to adjacent, organically managed croplands. Data on the frequency of occurrence and concentrations of pesticide residues in soil demonstrate a discrepancy between the determination of persistence and subsequent approval and their actual fate in soil. This raises the question of whether degradability of individual organic compounds has been adequately studied. Microbiological degradation is the most important process for reducing pesticide loads in soils. A reliable estimate of pesticide residence time requires an expanded understanding of the factors limiting microbial degradation. Degradation of anthropogenic organic chemicals in soils occurs much more slowly than would be expected based on their physicochemical properties. While processes that determine the fate of pesticides in soil have often been studied at different spatial and temporal scales, reasons for discrepancies between the observed complete degradation of pesticides under laboratory conditions and their persistence in the field remain unclear. This thesis addresses this challenge by focusing on the central question of why inherently biodegradable compounds in soils display increased persistence under field conditions. Organic contaminants in low but detectable and environmentally significant concentrations could remain in the soil once available concentrations fall below a threshold where bioenergetic growth restrictions come into play. In addition, potential microbial and biophysical limitations and environmental factors such as soil temperature and soil moisture are often examined separately in current degradation studies. Combinations of temperature and soil moisture changes associated with different concentration levels have been less well examined, resulting in an incomplete understanding of the degradation process. Another key factor in the demonstrated discrepancy between predicted and actual persistence in the field could be due to laboratory experiments that cannot account for field-scale processes. Therefore, degradation rates determined in laboratory experiments cannot be confidently extrapolated to the field scale. . This thesis identified further important regulatory mechanisms for microbially mediated pesticide degradation. The previously unknown concentration-dependent degradation dynamics and the concentration-dependent influence of limiting environmental conditions on microbial degradation emphasize the importance of studies using a realistic concentration range. Evidence of deep transport of a highly sorptive pesticide such as glyphosate primarily via preferential flow pathways into the subsoil with lower degradation dynamics underscores the need to include processes that can only be verified in field studies as part of risk assessments. The results of this thesis suggest that the biodegradation rates of pesticides are not homogeneous at field scales and may account in part for the discrepancy between complete degradation of pesticides under laboratory conditions and their persistence in the field. Laboratory studies in which soil samples are pooled and mixed to obtain a single "representative" sample can provide a simplified understanding of the process, but the complexity, particularly that of soil heterogeneity, of pesticide distribution and microbial degradation associated with prevailing climatic conditions, requires calibration of previously used methods in field studies and possibly at landscape, watershed, or regional scales. The scale-dependent degradation aspect will become even more important in the future; as soil properties and processes that control the toxicological aspects of contaminants include temperature and moisture, and changes associated with climate change will lead to an increase in extreme precipitation, longer dry periods, and soil erosion.
Modeling microbial regulation of pesticide turnover in soils
(2022) Chavez Rodriguez, Luciana; Streck, Thilo
Pesticides are widely used for pest control in agriculture. Besides their intended use, their long-term fate in real systems is not well understood. They may persist in soils, thereby altering ecosystem functioning and ultimately affecting human health. Pesticide fate is assessed through dissipation experiments in the laboratory or the field. While field experiments provide a close representation of real systems, they are often costly and can be influenced by many unknown or uncontrollable variables. Laboratory experiments, on the other hand, are cheaper and have good control over the governing variables, but due to simplification, extrapolation of the results to real systems can be limited. Mechanistic models are a powerful tool to connect lab and field data and help us to improve our process understanding. Therefore, I used mechanistic, process-based models to assess key microbial regulations of pesticide degradation. I tested my model hypotheses with two pesticide classes: i) chlorophenoxy herbicides (MCPA (2-methyl-4-chlorophenoxyacetic acid) and 2,4-D (2,4-Dichlorophenoxyacetic acid)), and ii) triazines (atrazine (AT)), in an ideal scenario, where bacterial degraders and pesticides are co-localized. This thesis explores some potential controls of pesticide degradation in soils: i) regulated gene expression, ii) mass-transfer process across the bacterial cell membranes, iii) bioenergetic constraints, and iv) environmental factors (soil temperature and moisture). The models presented in this thesis show that including microbial regulations improves predictions of pesticide degradation, compared to conventional models based on Monod kinetics. The gene-centric models achieved a better representation of microbial dynamics and enable us to explore the relationship between functional genes and process rates, and the models that used transition state theory to account for bioenergetic constraints improved the description of degradation at low concentrations. However, the lack of informative data for the validation of model processes hampered model development. Therefore, in the fourth part of this thesis, I used atrazine with its rather complex degradation pathway to apply a prospective optimal design method to find the optimal experimental designs to enable us identifying the degradation pathway present in a given environment. The optimal designs found suggest to prioritize determining metabolites and biomass of specific degraders, which are not typically measured in environmental fate studies. These data will lead to more robust model formulations for risk assessment and decision-making. With this thesis, I revealed important regulations of pesticide degradation in soils that help to improve process understanding and model predictions. I provided simple model formulations, for example the Hill function for gene expression and transition state theory for bioenergetic growth constraints, which can easily be integrated into biogeochemical models. My thesis covers initial but essential steps towards a predictive pesticide degradation model usable for risk assessment and decision-making. I also discuss implication for further research, in particular how mechanistic process-based modeling could be combined with new technologies like omics and machine learning.