Browsing by Subject "Landsorte"

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Gene mining in doubled haploid lines from European maize landraces with association mapping
(2014) Strigens, Alexander Carl Georg; Melchinger, Albrecht E.
Since the introduction of maize into Europe, open-pollinated varieties of flint maize were cultivated across the continent. Natural selection promoted adaptation to the climatic conditions prevailing in the different regions. With the advent of hybrid breeding in Europe during the 1950’s, some of the genes responsible for the specific adaptations of the landraces to abiotic and biotic stress were captured in the first developed inbred lines, but most of their genetic diversity is still untapped. Development of inbred lines out of this material by recurrent selfing is very tedious due to strong inbreeding depression. In contrast, the doubled-haploid (DH) technology allows producing fully homozygous lines out of landraces in only one step. This allows their precise characterization in replicated trials and identification of new genes by genome wide association (GWA) mapping. In this study we genotyped a set of 132 DH lines derived from European Flint landraces and 364 elite European flint (EU-F), European dent (EU-D) and North-American dent (NA-D) inbred lines with 56,110 single nucleotide polymorphism (SNP) markers. The lines were evaluated in field trials for morphologic and agronomic traits and GWA mapping was performed to identify underlying quantitative trait loci (QTL). In particular, our objectives were to (1) develop a robust method for quantifying early growth with a non-destructive remote-sensing platform, (2) evaluate the importance of early growth performance of inbred lines with regard to their testcross performance, (3) determine the potential of GWA mapping to identify genes underlying early growth and cold tolerance related traits, (4) evaluate the phenotypic and genotypic diversity recovered in the DH lines derived from the landraces, (5) estimate the effect of the DH method on the recovered genetic diversity, (6) identify new genes by GWA mapping in the DH lines derived from landraces, and (8) discuss the potential of DH lines derived from landraces to improve the genetic diversity and performance of elite maize germplasm. A phenotyping platform using spectral reflectance and light curtains was used to perform repeated measurements of biomass and estimate relative growth rates (RGR) of the DH and inbred lines, as well as of two testcrosses of 300 dent inbred lines. The DH lines derived from the landraces Schindelmeiser and Gelber Badischer had the highest RGR followed by EU-F lines, DH lines derived from Bugard, EU-D lines and, finally, NA-D lines. For inbred lines, whole plant dry matter yield (DMY) was positively correlated with RGR (r = 0.49), whereas this relation was weaker in the testcrosses (r = 0.29). RGR of the inbred lines correlated with RGR of their testcrosses (r = 0.42), but it had no influence on testcross DMY. A set of 375 EU-F, EU-D and NA-D lines were further evaluated in growth chambers under chilling (16/13°C) and optimal (27/25°C) temperatures. Photosynthetic and early growth performance were estimated for each treatment and an adaptation index (AI) built as the chilling to optimal performance ratio. Nineteen QTL were identified by GWA mapping for trait performance and AI. Candidate genes involved in ethylene signaling, brassinolide, and lignin biosynthesis were found in their vicinity. Several QTL for photosynthetic performance co-located with previously reported QTL and the QTL identified for shoot dry wieght under optimal conditions co-located with a QTL for RGR. Comparison of the DH lines derived from landraces with the EU-F lines showed that genotypic variances in single DH populations were greater than in the EU-F breeding population. A high average genetic distance among the DH lines derived from the same landrace as well as a rapid decay of linkage disequilibrium suggests a high effective population size of the landraces. Because no systematic phenotypic differences were observed between the landraces and synthetic landraces obtained by intermating the corresponding DH lines, the expected purge of lethal recessive alleles during the DH production did neither improve grain yield performance nor affect the recovered genetic diversity. Performing GWA in the DH lines derived from landraces as well as the EU-F, and EU-D lines allowed the identification of 49 QTL for 27 traits. A larger set of DH lines derived from more landraces might solve problems arising from population structure and allow a much higher power for the detection of new alleles. In conclusion, the introgression of DH lines derived from landraces into the elite breeding material would strongly broaden its genetic base. However, grain yield performance was 22% higher in EU-F lines than in the DH lines derived from landraces. Selection of the best DH lines would allow partially bridging this yield gap and marker-assisted selection may allow introgression of positive QTL without introducing negative features by linkage drag.
Preharvest and postharvest factors affecting the quality profile of onion landraces
(2021) Romo-Perez, Maria Luisa; Zörb, Christian
Onion cultivation has been practiced for over 4000 years and does not exist as a wild species. Over time onions have adapted to different climates, creating a wide range of varieties and landraces. Today, in modern agriculture, commercial onion breeders focus almost exclusively on conventional farming which increases the demand for certain well-known varieties and hybrids but lowers the diversity available in the mainstream market. Additionally, a clear need for new varieties of onions is heralded by organic farming systems, where pesticides and chemical fertilizers are banned. A way to preserve biodiversity and enrich the range of varieties available in organic farming systems is to re-evaluate traditional landraces and introduce their benefits to the broader public. Onions are known for their good storability, particular aroma, as well as for their health-promoting benefits due to the rich content of non-structural carbohydrates, flavonoids, and S-containing compounds. However, the quality status and sensorial characteristics of onions can be influenced through preharvest and postharvest factors. Some of those factors are genotype, soil, and storage conditions. Preharvest abiotic factors such as soil salinity can lead to several reduction of yield and quality aspects. Much like many other vegetable species, onions have always been classified as salt-sensitive crop. However, to date, there was very little evidence to that claim, and information about the impact of salinity on onion quality and physiology is lacking. This thesis aims to characterize onion landraces and compare them using targeted and untargeted metabolomics with commercial cultivars when grown under organic farming conditions. A part of this is evaluating the differences of landrace metabolite profile and the storage impact after five months of cold storage. Furthermore, this thesis discusses the effect of increased soil salinity on the metabolism and physiology of onion plants. In chapter 2, yield and quality aspects of studied onion varieties demonstrated that landraces can achieve similar or even better results than modern varieties Sturon and Red Baron when grown under organic farming conditions of South-West Germany. Furthermore, differences between Sturon and landraces demonstrated that parts of the aromatic and flavor properties found in landraces have been lost in modern genotypes (Chapter 3). These results indicate that the maximum potential of the modern onion varieties has not yet been reached and further optimization of their yield and quality parameter could be attained through future breeding programs that include local landraces. Among the studied landraces, Birnförmige, Stunova, and Rijnsburger 4 are the most interesting and promising candidates. For instance, Birnformige demonstrated not only good storability but also high levels of S-containing compounds and fructans. Stunova presented good yield stability and capacity, while Rijnsburger 4 exhibited the highest levels of amino acids suitable as precursors of aromatic substances as well as good storability. Despite several reports claiming that onions are sensitive to salinity, chapter 4 of this thesis demonstrated there is no reduction in plant growth, quality, or aroma in onion plants after moderate Na+ treatments. Nevertheless, in comparison with the landrace Birnformige, modern variety Sturon showed a slight increase of compatible solutes by Na+ accumulation, demonstrating that the potential of certain varieties for onion production under increased soil salinity is actually much higher than previously assumed.