Browsing by Subject "Lipidomics"

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Characterization of epidermal bladder cells in Chenopodium quinoa
(2021) Otterbach, Sophie L.; Khoury, Holly; Rupasinghe, Thusitha; Mendis, Himasha; Kwan, Kim H.; Lui, Veronica; Natera, Siria H. A.; Klaiber, Iris; Allen, Nathaniel M.; Jarvis, David E.; Tester, Mark; Roessner, Ute; Schmöckel, Sandra M.
Chenopodium quinoa (quinoa) is considered a superfood with its favourable nutrient composition and being gluten free. Quinoa has high tolerance to abiotic stresses, such as salinity, water deficit (drought) and cold. The tolerance mechanisms are yet to be elucidated. Quinoa has epidermal bladder cells (EBCs) that densely cover the shoot surface, particularly the younger parts of the plant. Here, we report on the EBC's primary and secondary metabolomes, as well as the lipidome in control conditions and in response to abiotic stresses. EBCs were isolated from plants after cold, heat, high‐light, water deficit and salt treatments. We used untargeted gas chromatography–mass spectrometry (GC–MS) to analyse metabolites and untargeted and targeted liquid chromatography‐MS (LC–MS) for lipids and secondary metabolite analyses. We identified 64 primary metabolites, including sugars, organic acids and amino acids, 19 secondary metabolites, including phenolic compounds, betanin and saponins and 240 lipids categorized in five groups including glycerolipids and phospholipids. We found only few changes in the metabolic composition of EBCs in response to abiotic stresses; these were metabolites related with heat, cold and high‐light treatments but not salt stress. Na+ concentrations were low in EBCs with all treatments and approximately two orders of magnitude lower than K+ concentrations.
Furan fatty acid amounts and their occurrence in triacylglycerols of white asparagus (Asparagus officinalis) from the German market
(2023) Müller, Franziska; Bauer, Vanessa; Vetter, Walter
Vegetables including asparagus contain a wide range of fatty acids, mainly stored in triacylglycerols. One class of interesting minor fatty acids is the family of furan fatty acids (FuFAs) because of their antioxidant properties. Since FuFAs have not been studied previously in asparagus (Asparagus officinalis L.), we developed and applied a simplified method for their analysis in 20 fresh and three preserved samples. Four FuFAs were detected with clear dominance of the dimethyl‐substituted FuFAs (D‐FuFAs) 11D5 and 9D5 as well as small amounts of the monomethyl‐substituted FuFAs (M‐FuFAs) 11M5 and 9M5. The total amounts of FuFAs in fresh white asparagus ranged from 1.4 to 4.6 mg/100 g dry weight (mean 3.0 mg/100 g dry weight). Subsequent LC‐Q‐Orbitrap‐HRMS measurements enabled the detection of 22 different FuFA‐containing TAGs. These were predominantly found together with one or two polyunsaturated fatty acid.