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Publication Bio-effectors for improved growth, nutrient acquisition and disease resistance of crops(2017) Weinmann, Markus; Neumann, GünterRecent scientific approaches to sustain agricultural production in face of a growing world food demand, limited natural resources, and ecological concerns have been focusing on biological processes to support soil fertility and healthy plant growth. In this context, the use of “bio-effectors”, comprising living (micro-) organisms and active natural compounds, has been receiving increasing attention. In contrast to conventional fertilizers and pesticides, the effectiveness of “bio-effectors” is essentially not based on the substantial direct input of mineral plant nutrients, neither in inorganic nor organic forms, nor of a-priori toxic compounds. Their direct or indirect effects on plant performance are rather based on the functional implementation or activation of biological mechanisms, in particular those interfering with soil-plant-microbe interactions. The general objective of the present research work was to improve the empirical and conceptual understanding concerning the utilization of bio-effectors in agricultural practice, following the principles of plant growth stimulation, bio-fertilization and bio-control. One main aspect of investigation was the application of bio-effectors to improve the efficiency of phosphorus (P) acquisition by the plant. Promising bio-preparations based on microbial inoculants (e.g. Bacillus, Pseudomonas, Trichoderma species) as well as natural compounds (e.g. algae extracts, humic acids) were tested in screening assays, greenhouse, and field experiments to characterize their potential effectiveness under varying environmental conditions. The most significant effects on plants appeared under severely low phosphate availability, but even under controlled conditions, bio-effectors required a narrow range of conductive environmental settings to reveal their potential effectiveness. Another focus of research was the application of bio-effectors to control soil borne pathogens, which typically appear in unsound crop rotations. Emphasis was set on take-all disease in wheat induced by the fungus Gaeumannomyces graminis. While the effectiveness of oat precrops to control take-all in subsequent wheat has been attributed to microbial changes and enhanced manganese (Mn) availability in soils, the take-all fungus is known to decrease the availability of Mn by oxidation. Against this background, the effectiveness of oat precrops and alternative crop management strategies to improve the Mn status and suppress the severity of take-all in wheat was investigated under controlled and field conditions. In conclusion, none of the tested supplemental treatments, such the application of microbial bio-effectors, stabilized ammonium or manganese fertilizers, could fully substitute for the multiple effectiveness of oat precrops, which was further confirmed by the results of a field experiment. Finally, some general conclusions and perspectives are summarized. Selected bio-effectors showed a strong capacity to improve the nutrient acquisition and healthy growth of crop plants under controlled conditions, but not in field experiments. However, even under controlled conditions the strongest effects occurred when plants were exposed to abiotic or biotic stresses, such as severely limited P availability or pathogen infestation of the soil substrate, still restricting plant growth to unproductive levels. Facing this situation, there is no perspective to improve the field efficiency of promising bio-effectors applications as a stand-alone approach. The only chance to develop viable alternatives to the conventional use of fertilizers or pesticides, for an ecological intensification of agriculture that maintains high yield levels, seems to be a reasonable integration of bio-effectors into the whole crop management of sound agricultural practice.Publication Bio-effectors for improved growth, nutrient acquisition and disease resistance of crops.- 2nd unrevised edition(2019) Weinmann, Markus; Madora GmbH, Luckestr.1, D-79539 Lörrach; Raupp, Manfred G.Recent scientific approaches to sustain agricultural production in face of a growing world food demand, limited natural resources, and ecological concerns have been focusing on biological processes to support soil fertility and healthy plant growth. In this context, the use of “bio-effectors”, comprising living (micro-) organisms and active natural compounds, has been receiving increasing attention. In contrast to conventional fertilizers and pesticides, the effectiveness of “bio-effectors” is essentially not based on the substantial direct input of mineral plant nutrients, neither in inorganic nor organic forms, nor of a-priori toxic compounds. Their direct or indirect effects on plant performance are rather based on the functional implementation or activation of biological mechanisms, in particular those interfering with soil-plant-microbe interactions. The general objective of the present research work was to improve the empirical and conceptual understanding concerning the utilization of bio-effectors in agricultural practice, following the principles of plant growth stimulation, bio-fertilization and bio-control. One main aspect of investigation was the application of bio-effectors to improve the efficiency of phosphorus (P) acquisition by the plant. Promising bio-preparations based on microbial inoculants (e.g. Bacillus, Pseudomonas, Trichoderma species) as well as natural compounds (e.g. algae extracts, humic acids) were tested in screening assays, greenhouse, and field experiments to characterize their potential effectiveness under varying environmental conditions. The most significant effects on plants appeared under severely low phosphate availability, but even under controlled conditions, bio-effectors required a narrow range of conductive environmental settings to reveal their potential effectiveness. Another focus of research was the application of bio-effectors to control soil borne pathogens, which typically appear in unsound crop rotations. Emphasis was set on take-all disease in wheat induced by the fungus Gaeumannomyces graminis. While the effectiveness of oat precrops to control take-all in subsequent wheat has been attributed to microbial changes and enhanced manganese (Mn) availability in soils, the take-all fungus is known to decrease the availability of Mn by oxidation. Against this background, the effectiveness of oat precrops and alternative crop management strategies to improve the Mn status and suppress the severity of take-all in wheat was investigated under controlled and field conditions. In conclusion, none of the tested supplemental treatments, such the application of microbial bio-effectors, stabilized ammonium or manganese fertilizers, could fully substitute for the multiple effectiveness of oat precrops, which was further confirmed by the results of a field experiment. Finally, some general conclusions and perspectives are summarized. Selected bio-effectors showed a strong capacity to improve the nutrient acquisition and healthy growth of crop plants under controlled conditions, but not in field experiments. However, even under controlled conditions the strongest effects occurred when plants were exposed to abiotic or biotic stresses, such as severely limited P availability or pathogen infestation of the soil substrate, still restricting plant growth to unproductive levels. Facing this situation, there is no perspective to improve the field efficiency of promising bio-effectors applications as a stand-alone approach. The only chance to develop viable alternatives to the conventional use of fertilizers or pesticides, for an ecological intensification of agriculture that maintains high yield levels, seems to be a reasonable integration of bio-effectors into the whole crop management of sound agricultural practice.Publication Entwicklung innovativer Pflanzenschutzprodukte und -verfahren als umweltfreundliche Alternativen zur Bekämpfung von Mehltaupilzen : Bericht im Rahmen des Forschungsprojektes: „Silizium als Aktivator bei Kulturpflanzen“(2019) Raupp, Manfred G.; Weinmann, Markus; Arbeitsgemeinschaft industrieller Forschungsvereinigungen „Otto von Guericke“ e. V. (AiF) Projekt GmbH, Berlin; Madora GmbH, Lörrach; Römheld, Volker; Neumann, Günter; Blaich, Rolf; Merkt, NikolausPowdery mildews are among the most important diseases in many crop plants. In all sectors of crop production (agriculture, viticulture, horticulture and orchards) powdery mildew fungi can cause severe damage under field as well as greenhouse conditions. Although organic synthetic fungicides have been used to combat powdery mildews in conventional and integrated agriculture for decades, organic farming lacks effective alternatives to the ecologically questionable sulfur fungicides. Yet, also for integrated or conventional crop production, alternatives or supplements for a reduction and more effective use of synthetic fungicides would be desirable to optimize the production of high quality food with the help of environmentally friendly means. Objective of the present work was the development of innovative crop protection products and application strategies to combat powdery mildew fungi with respect to the knowledge on resistance-enhancing effects of an improved silicon (Si), manganese (Mn) and zinc (Zn) supply to the plants. Furthermore, various plant extracts have recently received renewed attention. Among other active natural agents, garlic (Allium sativum L.) is known for its fungitoxic effect and at the same time high Mn and Zn contents. With the present work, an overview of possible approaches to control powdery mildew in grapevine by use of Si, Mn, Zn and plant extracts from garlic has been elaborated in greenhouse experiments. In this regard, the physiological significance of Si, Mn and Zn for the expression and strengthening of plant own resistance mechanisms was distinguished from the effectiveness of spray applications for forming passive silicate crusts as mechanical infection barriers. The physiological Si status of the plants could be clearly improved only by soil rather than foliar application of silicates. Regarding the soil application of silicates, however, no practical applications are known, how silicon fertilizers can be distributed under field conditions in the soil and brought into the rhizosphere to continuously ensure high rates of Si uptake. There is also still considerable uncertainty whether the soil application of silicates in non-Si accumulators, such as grapevines, can result in sufficient Si uptake for an effective expression of resistance mechanisms. The most impressive effects in the control of powdery mildew were achieved with the spray application of potassium silicate in combination with wetting agents to form silicate crusts on the leaf surface. The positive influence of Mn and Zn on the effectiveness of spray applications of potassium silicate and the adequate compatibility of Mn and Zn chelates with potassium silicate suggest that the interactions between Si, Mn and Zn should be considered for further product development. The application of garlic extract did not result in sufficient efficiency, although protective and curative properties could be observed. Allicin, supposed to be the active ingredient of garlic extract, has a broad spectrum of antimicrobial activity and is one of the few agents for which no development of resistance has been found in microorganisms so far. Therefore, the interest in this agent for the development of biological plant protection products is expected to increase.