Browsing by Subject "Mung bean"

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Evaluating suitable low-cost agar substitutes, clarity, stability, and toxicity for resource-poor countries' tissue culture media
(2022) Ebile, Pride Anya; Opata, John; Hegele, Suputtra
Over 20% of plant species assessed are threatened with extinction. Most of these plants have food security implications, especially in developing countries. Traditional seeds and cutting propagation techniques cannot counter the loss rate, and tissue culture provides a fast alternative to conventional propagation techniques. However, tissue culture has been considered too expensive for developing countries negatively impacted by food insecurity. A gelling agent is the costliest media component in plant tissue culture. This study aims to assess different gelling agents to find suitable ones with low cost and acceptable gelling properties for developing countries, especially in rural areas. Plantain explants were propagated on 16 starch-based substrates to evaluate their suitability as tissue culture gelling agents. This study compared the cost of various substrates and their gelling properties, such as clarity, toxicity, and texture, with agar as a reference gelling agent. Some substrates, such as xanthan, had good gelling properties, but their cost was too high (5.98 Euro L −1 ) to be considered low-cost. Other substrates, such as cassava starch, did not have suitable gelling properties; however, the cost was low (0.99 Euro L −1 ). Two of the substrates, mung bean and Isabgol, had suitable gelling properties and cost less than one euro. Therefore, smallholder banana and plantain farmers in resource-poor countries can undertake tissue culture operations with mung bean and Isabgol as gelling agents with minimum cost.
Optimizing mung bean productivity and root morphology with biofertilizers for sustainable farming
(2025) Nabati, Jaafar; Mirzaeetalarposhti, Reza; Yousefi, Afsaneh; Kurdestani, Ali Malakshahi; Nabati, Jaafar; Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran; Mirzaeetalarposhti, Reza; Institute of Crop Science, University of Hohenheim, 70599, Stuttgart, Germany
The excessive use of chemical fertilizers has raised major environmental and economic concerns in legume cultivation. This study assessed the effects of various fertilizers, including biofertilizers and chemical nitrogen, on yield, root characteristics, and nutrient dynamics in two mung bean genotypes (Partow, IC418452). Field trials were conducted over a two-year period in Mashhad, Iran, using a factorial randomized block design. Treatments included two genotypes and six fertilizer levels: control, N-fixing bacteria (FLNF), P-solubilizing bacteria (PSB), K-solubilizing bacteria (KSB), a consortium (FLNF + PSB + KSB), and Urea. Yield components, biomass, root morphology, nodulation, and plant/soil NPK concentrations were measured and analyzed. Fertilizers significantly affected yield, biomass, root structure, and nutrient uptake. Urea yielded the highest biomass, grain yield, and root area, especially in Partow. However, the microbial consortium significantly improved yield components compared to the control and uniquely maximized root nodulation and inoculation percentage, indicating an enhanced biological nitrogen fixation potential. On average across the two seasons, urea increased grain yield by 46% and biomass by 41% relative to the control, whereas the microbial consortium enhanced root nodulation by 62% and yield by 32%. Significant genotype×fertilizer interactions highlighted genotype-specific responses. Nodulation correlated positively with yield. Both urea and the PGPR consortium significantly increased mung bean productivity. While urea maximized yield, the consortium provided considerable yield gains and enhanced biological nitrogen fixation potential, presenting a viable and sustainable alternative to reduce reliance on chemical nitrogen. The goal was to understand the relationships between root morphology, nutrient utilization, and yield to promote sustainable, high-yield mung bean cultivation. These findings highlight the potential of multi-strain biofertilizers to maintain mung-bean productivity while reducing dependence on synthetic N inputs.