Browsing by Subject "Radiation COX-2 signalling pathways"

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Analyse relevanter Signalwege der strahleninduzierten COX-2 Expression in Tumorzellen
(2007) Krebiehl, Guido Klaus; Rodemann, H.-Peter
Summary: Cancer is a health problem worldwide and the number of new cases is rising. Surgery, radiotherapy and chemotherapy are the major treatment modalities. New developments in radiotherapy make radiation alone and in combination with chemotherapy to an important therapy becoming more and more mattering. The success of a therapy often depends on the genetic profile of a tumor. This makes analysis of molecular processes in cells after radiation an important aspect in radiotherapy developing an effective strategy for tumor treatment. COX-2 is overexpressed in a lot of tumors and correlates with a poor prognosis. Moreover COX-2 can be induced by ionizing radiation. This makes COX-2 an interesting molecular target in radiation therapy and in cancer therapy in general. Studies with specific COX-2 inhibitors came to different results in different cell lines. The aim of the presented study was to investigate the survival and the proliferation of prostate cancer cells after treatment with ionizing radiation alone and in combination with specific COX-2 inhibitor Celecoxib and the analysis of signaling pathways leading to radiation induced COX-2 expression. The following major results were obtained: 1. Treatment with Celecoxib had no influence on the radiosensitivity of the prostate cancer cell lines investigated. 2. The proliferation of different cell lines was inhibited by the treatment with Celecoxib. 3. The inhibition of the proliferation seems to be independent of the level of COX-2 of the cell lines. 4. Apoptosis can not be induced by Celecoxib in clinical relevant doses in the cell lines investigated. 5. Induction of COX-2 expression by ionizing radiation depends on the cell line investigated. 6. The MAPK-signaling pathways play a major role at COX-2 expression. In conclusion the results of the presented study indicate that COX-2 can be an important molecular target in radiation therapy. Although this depends on the cell line investigated. As well, the signaling pathways leading to a radiation induced expression of COX-2 are individual for each cell line. Thus the application of Celecoxib during radiation therapy can be positive on the treatment of different tumors.