Browsing by Subject "Stickstoffkreislauf"

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Biochar amendment for C sequestration in a temperate agroecosystem : implications for microbial C- and N-cycling
(2018) Bamminger, Chris; Kandeler, Ellen
Climate warming will have great impact on terrestrial ecosystems. Different soil properties such as temperature and moisture will be altered, thereby influencing C- and N-cycles, microbial activity as well as plant growth. This may contribute to the observed increase in soil greenhouse gas (GHG) emissions under climate change. Therefore, new options are needed to mitigate theses projected consequences. Biochar is primarily suggested to be effective in long-term C sequestration in agricultural soils due to its long-term stability. In addition, it could be applied to improve various soil properties, plant growth and to reduce soil GHG emissions. To date, knowledge about such beneficial biochar effects in soil under predicted warming climate is extremely scarce. In the first study, a slow-pyrolysis biochar from Miscanthus x giganteus feedstock (600 °C, 30 Min.) was incubated for short time (37d) under controlled laboratory conditions in agricultural soil in the presence of earthworms and N-rich litter (Phacelia tanacetifolia Benth.). Biochar increased microbial abundances and the fungal-to-bacterial PLFA ratio after 37 days in arable soil applied with litter suggesting improved living conditions for microorganisms with biochar. Fungi may benefit most from newly created habitats due to colonizable biochar pores and surfaces. Additionally, fungi could have also mineralized small amounts of recalcitrant biochar-C during plant litter decomposition. Without litter, biochar led to interactions between earthworms and soil microorganisms resulting in enhanced bacterial and fungal abundances. This indicates better growth habitats for soil microbes in earthworm casts containing biochar. Soil respiration and metabolic quotients (qCO2) and N2O emissions (in litter treatments) were decreased after biochar application suggesting a more efficient microbial community and underscoring the GHG mitigation potential of the used biochar. The field experiment, investigated in the second and third study, focused on the stability and long-term soil C sequestration potential of comparable Miscanthus biochar (850 °C, 30 Min.). Related effects on soil GHG emissions, physical, chemical and microbiological soil properties as well as plant growth were determined in an agroecosystem at year-round elevated soil temperature (+2.5 °C, since 2008). The second study investigated the short-term effects of biochar on microbial abundances and growth of winter rapeseed during the first year after field application to a warmed temperate arable soil. It was found that fungal biomass and the fungal-to-bacterial ratio were increased in the warmed biochar plots only after three months in the presence of spring barley litter from the previous growing season. The disappearance of this effect points to an overall high stability of the investigated biochar. Moreover, biochar proved to be effective in mitigating negative effects of seasonal dryness on microbial abundances and early plant growth in the dry spring period in 2014. However, biochar had no effect on final aboveground biomass of winter rapeseed at harvest in the first growing season. As shown in the third study, after two vegetation periods of winter rapeseed and spring wheat, the assumption that plant productivity in already fertile temperate arable soils is unlikely to be further enhanced with biochar amendment, was confirmed. Total CO2 emissions after two years were not reduced by biochar and remained unchanged even under warming suggesting a high degradation stability of the used biochar. N2O emissions were increased in biochar-amended soil at elevated soil temperature, presumably due to enhanced water and fertilizer retention with biochar. By using the global warming potential (GWP100) of total soil GHG emissions, the storage of biochar-C in soil was estimated to compensate warming-induced elevated soil GHG emissions for 20 years. To conclude, this thesis revealed that biochar may have only minor influence on soil microorganisms and crop growth in temperate, fertile arable field soils. However, it was shown that biochar could be a valuable tool for C sequestration in temperate arable soils, thus potentially offsetting a warming-induced increase in GHG emissions. In order to face climate change impacts, more long-term studies on microbiological effects and the C sequestration potential of biochar in cultivated soil under warming are urgently needed.
Developing indicators and characterizing direct and residual effects of biological nitrification inhibition (BNI) by the tropical forage grass Brachiaria humidicola
(2018) Karwat, Hannes; Cadisch, Georg
Nitrogen (N) losses from agroecosystems harm the environment via increased nitrate (NO3-) amounts in water-bodies and nitrous oxide (N2O) emissions to the atmosphere. Bacteria and archaea oxidize ammonium (NH4+) to NO3- under aerobic conditions. Furthermore, under mainly anaerobic conditions, microbial denitrification reduces NO3- to gaseous N forms. The tropical forage grass Brachiaria humidicola (Rendle) Schweick (Bh) has been shown to reduce soil microbial nitrification via root derived substances. Therefore, biological nitrification inhibition (BNI) by Bh might contribute to reduction of N losses from agroecosystems. The present doctoral thesis aimed at assessing the potential of the actual BNI by Bh, as well as the residual BNI effect with new developed methodologies. The overall research was based on the following major objectives: (1) characterization of the residual BNI effect by Bh on recovery of N by subsequent cropped maize (Zea mays L.) under different N fertilization rates; (2) investigate if low enzymatic nitrate reductase activity (NRA) in leaves of Bh is linked to reduced NO3- nutrition by effective BNI; (3) identify a possible link between plant delta 15N of Bh and the BNI effect of different Bh genotypes on nitrification, plant N uptake and NO3- leaching losses. The overall objective was to use and test new methodologies with a minimum of disturbance of the plant-soil system, to characterize BNI of different Bh genotypes in greenhouse and field studies. The first research study focused on the investigation of a potential residual BNI effect of a converted long-term Bh pasture on subsequent maize cropping, where a long-term maize monocrop field served as control. The residual BNI effect was characterized in terms of enhanced maize grain yield, total N uptake and 15N (labeled) fertilizer recovery. Furthermore, the impact of residual BNI effect on soil N dynamics was investigated. The residual BNI effect was confirmed for the first maize crop season after pasture conversion on the basis of lower nitrification in incubation soil, higher total N uptake and higher maize grain yields. However, the residual BNI effect did not result in higher 15N fertilizer uptake or reduced 15N fertilizer losses, nor in reduced N20 emissions. Applied N was strongly immobilized due to long-term root turnover effects, while a significant residual BNI effect from Bh prevented re-mineralized N from rapid nitrification resulting in improved maize performance. A significant residual Bh BNI effect was evident for less than one year only. In the second research study it was the aim to verify the potential of nitrate reductase activity (NRA) as a proxy for the detection of in vivo performance of BNI by selected Bh accessions and genotypes grown under contrasting fertilization regimes. NRA was detected in Bh leaves rather than in roots, regardless of NO3- availability. Leaf NRA correlated with NO3- contents in soils and stem sap of contrasting Bh genotypes substantiating its use as a proxy of in vivo performance of BNI. The leaf NRA assay facilitated a rapid screening of contrasting Bh genotypes for their differences in in vivo performance of BNI under field and greenhouse conditions; but inconsistency of the BNI potential by selected Bh genotypes was observed. The third research study emphasized to link the natural abundance of delta 15N in Bh plants with reduced NO3- losses and enhanced N uptake due to BNI. Increased leached NO3- was positively correlated to rising delta 15N in Bh grass, whereas the correlation between plant N uptake and plant delta 15N was inverse. Long-term field cultivation of Bh decreased nitrification in incubated soil, whereas delta 15N of Bh declined and plant N% rose over time. Delta 15N of Bh correlated positively with assessed nitrification rates in incubated soil. It was concluded that decreasing delta 15N of Bh over time reflects the long-term effect of BNI linked to lower NO3- formation and reduced NO3- leaching, and that generally higher BNI activity of Bh is indicated by lower delta 15N plant values. Within the framework of this thesis, a residual BNI effect by Bh on maize cropping could be confirmed for one season due to the combined methodological approaches of soil incubation and 15N recovery. The development of the NRA assay for sampled Bh leaves was validated as a rapid and reliable method linked to the actual soil nitrification after NH4+ fertilizer supply. Consequently, the assay could be used for both greenhouse and field studies as BNI proxy. The gathered data from the third study indicated that decreasing delta 15N of Bh over time reflects the long-term effect of BNI linked to lower NO3- formation and reduced NO3- leaching, and that generally higher BNI activity of Bh is indicated by lower delta 15N plant values. Consequently, it was suggested that delta 15N of Bh could serve as an indicator of cumulative NO3- losses. Overall, this doctoral thesis suggests the depressing effect on nitrification by Bh might be a combined effect by BNI and fostered N immobilization. Furthermore, BNI by Bh might be altered by different factors such as soil type, plant age and root morphology of the genotypes. Finally, future studies should consider that Bh genotypes express their respective BNI potential differently under contrasting conditions.
Nitrogen dynamics in organic and conventional farming systems in the sub-humid highlands of central Kenya
(2019) Musyoka, Martha; Cadisch, Georg
Nitrogen (N) deficit is one of the limiting factors to food security in most developing countries while the excessive use of N has resulted in environmental contamination. Timely N availability, at the right rate is crucial to improving crop yield and N use efficiency in farming systems. Therefore, understanding nitrogen dynamics under different farming systems is essential to improve N use and recovery efficiencies of crops and in addressing environmental impacts associated with increased use of inorganic and organic inputs. This study focused on N dynamics in conventional (Conv) and organic (Org) farming systems as practiced by small scale farmers (at ∼50 kg N ha−1yr−1, Low input) and at recommended levels of input (∼225 kg N ha−1yr−1, High input) for commercial use in the sub humid and humid regions of Central Kenya. Data was collected during three cropping seasons between October 2012 and March 2014 in an on-going long-term trial established since 2007 at Chuka and at Thika sites located in central highlands of Kenya. Mineral N-based fertilizer and cattle manure were applied in Conv-High and Conv-Low while composts and other organic inputs were applied at similar N rates for Org-High and Org-Low. Farming systems were laid down in a randomized complete block design with 4 and 5 replications at Chuka and Thika respectively. The trial follows a 2 season-three-year crop rotation envisaging maize, legumes, vegetables and potatoes. N mineralization was studied using a modified buried bag approach while N loss was measured using Self-Integrating Accumulator (SIA) cores. N synchrony was assessed using daily N flux differences constructed as daily N release minus daily N uptake at different stages of the crops. N uptake was assessed at various stages of the crop through destructive sampling while nitrogen use efficiency (NUE) was assessed at harvest. Surface N balances were constructed using N applied as inputs, N deposition via rainfall, biological N fixation and crop yield and biomass as outputs. Out of the total N applied from inputs, only 61, 43 and 71 % was released during potato, maize and vegetable seasons respectively. Farming systems did not show a major impact in their influence on N synchrony, i.e. matching N supply to meet N demand. Rather the N synchrony varied with crop and N demand stages. Positive N flux differences were observed (higher N release compared to N demand) during the initial 20-30 days of incubation for all the farming systems, and negative N flux differences (higher N demand than release) at reproductive stages of the crops. Nitrogen uptake efficiency (NUpE) of potato was highest in Conv-Low and Org-Low at Thika and lowest in Org-High and Org-Low at Chuka where late blight disease affected potato performance. In contrast, NUpE of maize was similar in all systems at Chuka site, but was significantly higher in Conv-High and Org-High compared to the low input systems at Thika site. The NUpE of cabbage was similar in Conv-High and Org-High while the NUpE of kale and Swiss chard were similar in the low input systems. Potato N utilization efficiencies (NUtE) and agronomic efficiencies of N use (AEN) in Conv-Low and Conv-High were higher than those from Org-Low and Org-High, respectively. The AEN of maize was similar in all the systems at Chuka but was higher in the high input systems compared to the low input systems at the site in Thika. The AEN of vegetables under conventional systems were similar to those from organic systems. Both conventional and organic systems lost substantial amounts of mineral-N into lower soil horizons before crop establishment (0-26 days). Cumulative NO3--N leached below 1 m was similar in all the farming systems but was higher at the more humid Chuka site compared to Thika site during the maize season. Significantly more N was leached during potato season compared to maize and vegetable seasons. When NO3--N leached was expressed over total N applied, 63-68% more NO3--N was leached from the low input systems compared to the high input systems. Org-High showed a positive partial N balance at both sites and in all the cropping systems except during the vegetable season at Chuka. All the other systems exhibited negative partial N balances for the three cropping seasons with exception of Conv-High during potato season and Conv-Low and Org-Low during vegetable season at Thika site. In summary, organic and conventional had similar effects on N release, synchrony and N loss through leaching. Furthermore, more N was leached (when expressed as a fraction of N applied) during potato and vegetables cropping seasons in the low input systems compared to the high input systems. In addition, conventional and organic farming systems had similar effects on NUpE, AEN, NUtE and NHI for maize and vegetables, while conventional systems improved NUE of potato compared to organic systems. The research therefore concludes that organic and conventional farming systems at high input level are viable options of increasing food security in sub-Saharan Africa (SSA) for maize and vegetables as demonstrated by similar yields, NUE, N supply and loss. Ability to meet food security in conventional and organic system at low input is hampered by high N losses, negative N balances coupled with low productivity due to biotic and abiotic stresses. In both conventional and organic systems, there is a need to reduce N application at planting and increase N applied at reproductive stages to minimize potential loss during the initial 20-30 days after application and improve N supply midseason when crop demand is high. Since organic systems depend on organic inputs, there is a critical need to improve the quality of manure, composts and other organic inputs to improve N supply and availability.
Soil conservation methods and their impact on nitrogen cycling and competition in maize cropping systems on steep slopes in Northwest Vietnam
(2015) Vu Dinh, Tuan; Cadisch, Georg
Recent maize cultivation expansion into steep forested uplands in Vietnam led to severe erosion, soil degradation, and strong environmental impacts. Despite effectively controlling erosion, conservation measures often reduce crop yields due to resource competition. To foster uptake of soil conservation, a study including two experiments with bounded plots at two communes - Chieng Hac (21.02° N, 104.37° E, inclination: 53%) and Chieng Khoi (21.02° N, 104.32° E, inclination: 59%) - was carried out over a period of three years (2009-2911). The treatments included maize monocropping under intensive tillage and fertilization (T1, control), maize with Panicum maximum as grass barrier (T2), maize under minimum tillage (MT) with Pinto peanut (Arachis pintoi) as cover crop (T3), and maize under MT and relay cropped with Adzuki beans (Phaseolus calcaratus) (T4). Soil loss in 2010 and 2011 were also measured using sediment fences on unbounded maize fields under current farmers’ practice. The first part of the study assessed the magnitude of erosion and the mitigation potentiality of soil conservation measures. Under farmers’ practice, annual soil losses of bounded plots reached up to 174 t ha-1, being much higher than those from unbounded fields (up to 111 t ha 1). The majority of the soil loss occurred early in the season, when high rainfall intensities coincided with a low percent ground cover (<30%). To keep erosion rates below a tolerable soil loss (3 t/ha/yr) on steep slopes (53-59%) under an average annual rainfall of 1270 mm, a theoretical minimum ground cover of 95% is required at the onset of the crop season which was hardly achievable under monoculture system. Under conservation measures erosion was reduced by 39-84% in grass barriers or by 93-100% in MT with cover crops. A yield decline of 26% was observed in grass barrier treatments or up to 35% of cover crop plots if Pinto peanuts were not cut on time. Both options provided animal feed, up to 5.5 t/ha/yr dry matter of grass or 1.8 t/ha/yr dry matter of Pinto peanuts. Despite these potential benefits, constraints such as labour for grass barriers and cover crop establishment and cutting it afterwards, or difficulties in accessing and collecting maize cobs due to proliferate growth of Adzuki beans may hinder adoption by local farmers. To increase the incentive for adoption, the conservation system also has to use N fertilizer more efficiently. Therefore, the second part of the study examined the fate of applied 15N-labelled urea at the Chieng Hac site in 2010. At harvest, 21.6% of the labelled 15N was recovered by maize in T1, 8.9% in T2, 29% in T3, and 30.9% in T4. In T2, maize and P. maximum competed heavily for N with a total of 23.6% of the applied 15N found in the barriers next to application point. About 46-73% of the maize N uptake was derived from the soil, showing the important role of inherent soil N in these fertilized systems. MT reduced 15N translocation to deeper soil layers (40-80 cm), indicating a safety net function. Downslope translocation (>17 m) of applied 15N was <0.1 kg ha-1 as the majority of 15N added was vertically translocated and intercepted by plants along the slope. Despite implementation of an improved fertilization method, approximately 24-46% of N-fertilizer was unaccounted for, presumably lost via volatilization, denitrification, and leaching below 80 cm. Measured data for plot level showed that current farming practice (T1) induced a negative N balance of -142 kg N ha-1 in which residue burning and erosion were major pathways for N losses. A less severe negative N balance in T2 was attributed to reduced N losses by erosion while positive N balances of MT treatments were accredited to strongly reduced N losses via erosion and abandonment of burning plant residues in these treatments. The third part of the study investigated causes of competition in conservation systems three years after their establishment (2011). A pre-test at Chieng Hac in 2010 showed that abundance of water and the lack of N fertilization induced low grain N concentrations, enriched 15N;13C values in leaves, and reduced maize grain yield. This pattern was also observed in maize rows grown next to grass barriers or in cover crop plots at Chieng Khoi under good water availability conditions, indicating that these yield declines were mainly forced by lack of N. Additionally, a positive water balance throughout the maize cropping season further confirmed that water stress was absent. Moreover, enriched 15N values of maize rows close to the barriers suggested that these plants had to rely on soil N rather than on 15N derived from fertilizer N. Similarly, results of MT with simultaneous growth of A. pintoi pointed to N competition, resulting in a maize yield decline due to vigorous cover crop growth in T3. In contrast, MT with a relay crop (T4) had a similar maize yield, leaf N concentration, d15N, d13C as the control, suggesting N and water competition did not occur. In conclusion, soil erosion and nitrogen balances of current farming practice showed the urgent need to safeguard land resources, counteracting soil degradation but maintaining crop yields. The tested conservation techniques provide a range of characteristics to be considered as a sustainable system. The grass barrier as well as conservation systems controlled erosion, while minimum tillage with a cover crop further improved the nitrogen balance, and finally minimum tillage with a relay crop adds another advantage in maintaining crop production. Likelihood of adoption, however, may vary with how well appropriate incentives and land use policy fit to the area.