Browsing by Subject "Transgene Pflanzen"

Now showing 1 - 2 of 2

Genotypische Variation der Überdauerungsneigung von transgenem und konventionell gezüchtetem Raps und Möglichkeiten der Beeinflussung durch Bodenbearbeitung als Beitrag zur Sicherheitsforschung bei transgenen Kulturpflanzen
(2004) Gruber, Sabine; Claupein, Wilhelm
High losses during harvesting of oilseed rape in combination with secondary dormancy of the seeds can result in a large soil seed bank which may persist for several years. Volunteers emerging from this seed bank cannot be controlled completely, particularly when they develop in another rapeseed population. In addition to well known agricultural problems, the risk of temporal and spatial gene dispersal by persistent seeds and volunteers gets more significant. With regard to genetically modified (GM) cultivars, seed dormancy and persistence of oilseed rape volunteers have to be reconsidered. The aim of the current study was to investigate the chances for a reduction of seed persistence and gene dispersal by growing specific genotypes and by the implementation of appropriate tillage operations. Four publications describe and discuss experiments in the laboratory and the field on the aspects ?genotype? and ?soil tillage?. One central point was the characterisation of the genotypic variation of secondary dormancy and seed persistence in GM (herbicide tolerant) and conventionally bred oilseed rape. A gradual approach towards field conditions was performed by three experiments. The first experiment examined potential seed persistence by artificial induction of secondary dormancy in the laboratory. The second experiment was a burial of seeds in the soil for six months on a field. The third experiment examined persistence of seeds actually lost during harvest and exposed afterwards to different tillage operations in a field experiment. All three experiments showed a high genotypic variability in seed dormancy and persistence. In the laboratory the level of secondary dormancy of conventional cultivars was a total of 3?76% and of the GM cultivars 1?31%. The number of persistent seeds in the burial experiment was 7?90% in the conventional and 12?79% in the GM assortment. Seeds from the seed rain of the four conventionally bred cultivars in the field experiment persisted in the soil from 0?11% over six months. A significant, positive correlation was found between the laboratory results for cultivars from two crop years as well as between the results from laboratory and burial experiments. This is an indication for a genetic background of seed dormancy and persistence of oilseed rape. It has been demonstrated that a laboratory method for induction of secondary dormancy can describe differences of seed persistence in the field. This result gives a chance for screening new cultivars in the laboratory to identify desired, low persistent genotypes. The other focus of the study was the effect of various tillage treatments on seed persistence and seedling recruitment in the field. Four differently intensive tillage operations incorporated the seeds of two near-isogenic cultivars at different times and soil depths, or left the soil untilled. Winter wheat was sown as following crop in all treatments whereby no weed control was performed. The highest number of seeds generally entered the soil seed bank when seeds were incorporated into the soil by stubble tillage immediately. After six months 1?14% of the initial seed input was found again in the soil in these treatments, and in one isolated case about 28%. Delaying the stubble breaking, the soil seed bank was 0?3%. Leaving the seeds undisturbed on the soil surface until direct drilling resulted in a soil seed bank of 0?17%. Therefore, the first tillage operation after harvest of oilseed rape should be performed with some time delay to avoid large soil seed banks. Soil inversion by a mouldboard plough shifted the majority of seeds into deep soil horizons from where a successful germination was restricted. In contrast, primary tillage by a rigid tine cultivator or zero tillage mainly distributed the seeds within the upper soil layer. Thus the number of flowering volunteers in the first spring was highest in these treatments, with a maximum of one volunteer m-2. Gene dispersal from oilseed rape volunteers to other rape crops and feral relatives was possible because their flowering periods overlapped. The volunteers were able to produce viable seeds, despite high levels of damage by pests and diseases. Overall, a high genotypic variability was found for seed persistence of oilseed rape. Tillage operations, particularly the time of stubble tillage, can also influence seed persistence and the occurrence of volunteers. Combined with a thorough knowledge of the processes involved, the selection of low persistent genotypes and adequate tillage operations offer chances to limit or even to avoid undesired gene dispersal from oilseed rape volunteers, and to make gene dispersal a predictable factor.
Resistance of Maize (Zea mays L.) Against the European Corn Borer (Ostrinia nubilalis Hb.) and its Association with Mycotoxins Produced by Fusarium spp.
(2004) Magg, Thomas; Melchinger, Albrecht E.
The European corn borer (ECB, Ostrinia nubilalis Hübner) is a major pest of maize (Zea mays L.) in Europe and continues to spread to northern maize growing regions. The ECB severely affects commercial maize production by decreasing yield stability. In addition, damaged plants often show an increased susceptibility to secondary infections caused by Fusarium spp.. Information about the potential of Bt hybrids (event 176, MON810) to reduce yield losses and mycotoxin contamination under Central European growing conditions is still lacking. However, such monogenic resistances with a strong negative effect on the ECB will break down rapidly. Improving the natural host plant resistance of maize could provide an economical and ecological tool for an integrated pest management system. The overall goal of this study was to evaluate alternative breeding strategies for improving resistance of maize against ECB damage and Fusarium spp.. The objectives were to (1) initiate a selection experiment in the early maturing European flint pool and evaluate a breeding program for ECB resistance in the European dent pool, (2) compare the efficiency of host plant resistance vs. Bt resistance in maize, (3) determine Fusarium-caused mycotoxin contamination of maize genotypes with improved host plant resistance to ECB, and (4) study the association between important agronomic traits, ECB resistance traits, and mycotoxin concentration in early European maize germplasm. The goal of the Hohenheim ECB breeding program, initiated in 1992, was to select lines with improved per se and testcross performance for multiple agronomic traits and ECB resistance. In the standard breeding scheme, line development started from a segregating S1 population. Genotypes were evaluated for their line per se ECB resistance in generations S1, S3, and S5. Lines from the S2, S4, and S5 generations were testcrossed and evaluated for their agronomic performance. Selection was based on ECB resistance and TC performance for grain yield and maturity. In order to compare transgenic Bt maize hybrids carrying event 176 or MON810 with their isogenic counterparts and commercial hybrids or experimental hybrids, field trials in multiple environments were conducted in 1998 to 2000. Furthermore, a laboratory bioassay with neonate ECB larvae was performed to assess mortality and subsequently the level of Bt antibiosis present in the used hybrids of 1998. Resistance traits such as damage rating of stalks, number of damaged plants, and number of larvae per plant were assessed exclusively in manually ECB infested plots. Grain yield, grain dry matter content and plant height were determined in the insecticide protected and the ECB infested main plots. In addition, grain samples from each subplot were drawn at random and analyzed separately for Fusarium mycotoxins such as type B trichothecenes (DON, NIV), Zearalenon (ZEN), Fumonisins (FUM), and Moniliformin (MON). The inbred lines displayed a significant genotypic variance for all ECB resistance traits evaluated. However, in the further course of selection and topcross testing, most dent and flint lines, especially those displaying improved resistance to ECB larvae feeding, were discarded because of their poor agronomic performance. Negative correlations between grain yield, early maturity and the damage rating of stalks were identified. However, three dent lines (P028, P029, P030) with moderate resistance to ECB were developed. In all experiments, Bt hybrids were superior to other hybrids in the control of ECB larvae. Non-Bt hybrids displayed a significant genotypic variance for all evaluated resistance traits; grain yield reductions ranged from 8.6 to 21.8% under manual infestation of ECB. All evaluated resistance traits were highly significantly correlated with each other and showed significant negative correlations to grain yield reduction. Bt hybrids did not differ from their isogenic counterparts for most agronomic traits. Highly significant location and genotype × location interactions were identified for all mycotoxins evaluated, except MON. MON concentration doubled under manual infestation of ECB compared to insecticide protected conditions and a similar trend was found for FUM. Bt hybrids displayed significantly lower MON concentrations than non-Bt hybrids and significantly lower DON concentrations than their isogenic counterparts under ECB infestation. Highly significant correlations between ECB resistance traits and MON were found. However, a significant genotypic variance was observed for DON, 15-A-DON, FUM, and MON concentrations, suggesting variation for resistance against Fusarium spp. in current elite hybrids. By combining different sources of monogenic Bt resistance and quantitatively inherited resistances to ECB, it may be possible to develop hybrids with multiple resistance by pyramiding the underlying genes in one genotype. Therefore, further research is required to identify new sources of ECB resistance and new breeding strategies should be developed. Furthermore, there is indication that an improved resistance against Fusarium spp. possesses a greater potential for reducing mycotoxin contamination of maize kernels than a high level of ECB resistance. Since resistance to ECB and resistance to Fusarium spp. are inherited fairly independently, simultaneous improvement of both resistances seems to be necessary for improving the stability and quality of future maize hybrids.