Browsing by Subject "Wasserkopf"

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Die Rolle von hmmr während Neurulation und Hirnentwicklung im Afrikanischen Krallenfrosch Xenopus laevis
(2016) Hagenlocher, Cathrin; Schweickert, Axel
The cerebrospinal fluid (CSF) fills the entire ventricular system of the brain, the spinal cavity and the subarachnoid space. CSF mechanically buffers the brain, transports signaling molecules and eliminates waste products. It is produced by the choroid plexus (CP) and transported throughout the ventricular system via motile cilia. Excessive production, diminished transport or reduced absorption of CSF lead to hydrocephalus, a pathological dilatation of the brain ventricles. Mutations in humans and mice showed that dysfunctional and immotile cilia also induce hydrocephalus. The underlying mechanism through which disturbed ciliary motility leads to formation of hydrocephalus is not resolved. In the present thesis the model organism Xenopus laevis was used to analyze the occurrence of hydrocephalus upon on ciliary dysmotility. Biogenesis of motile cilia was described in the Xenopus laevis brain up to metamorphosis. Gene expression of foxj1, the superior regulator of the biogenesis of motile cilia, correlated with development of elongated monocilia and the switch to multiciliated ependymal cells. Cilia on foxj1-positive cells were motile and produced a directional flow of CSF. foxj1 loss-of-function led to impaired or absent motile cilia and resulted in hydrocephalus. The development of the hydrocephalic dilatation correlated with reduced velocity of the cilia-driven CSF-flow below 300 µm/s. In cilia of the airway epithelium regulation of ciliary beat frequency via HMMR has been described with HMMR loss-of-function resulting in reduced ciliary beat frequency. In line with these results, hmmr loss-of-function in Xenopus laevis resulted in reduced velocity of CSF-flow and hydrocephalus. This suggests that especially in the fourth ventricle CSF-flow velocities above 300 µm/s are necessary to maintain a homeostatic fluid pressure in the entire ventricular system. The loss-of-function of foxj1 as well as hmmr further led to severe malformations in the dorsal midline of the brain, especially of the CP and the subcommissural organ. These ciliated structures have already been connected to development of hydrocephalus. Brain defects after loss-of-function of hmmr reflected the human disorder of holoprosencephaly (HPE) which often results from mutations in the Shh-signaling pathway and leads to hydrocephalus. Interestingly after hmmr loss-of-function induced HPE was independent of the Shh-signaling pathway. Forebrain development was disturbed because hmmr was necessary for microtubule-mediated cell adhesion during the morphogenetic movements of neurulation. This study shows for the first time, that CSF in Xenopus laevis is transported via motile cilia and confirmes that dysfunction or absent motile cilia lead to congenital hydrocephalus. Furthermore a novel role for motile cilia during fore- and midbrain morphogenesis was demonstrated. Development of hydrocephalus together with forebrain defects in foxj1 and hmmr morphants implies that cilia-dependent hydrocephalus can result from malformed dorsal midline structures. This study thus provides a basis to establish Xenopus laevis as a model organism to study the development of hydrocephalus caused by primary cilia dyskinesia and by forebrain defects.