Surfactin shows relatively low antimicrobial activity against Bacillus subtilis and other bacterial model organisms in the absence of synergistic metabolites

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dc.contributor.authorLilge, Lars
dc.contributor.authorErsig, Nadine
dc.contributor.authorHubel, Philipp
dc.contributor.authorAschern, Moritz
dc.contributor.authorPillai, Evelina
dc.contributor.authorKlausmann, Peter
dc.contributor.authorPfannstiel, Jens
dc.contributor.authorHenkel, Marius
dc.contributor.authorMorabbi Heravi, Kambiz
dc.contributor.authorHausmann, Rudolf
dc.date.accessioned2024-10-23T12:25:46Z
dc.date.available2024-10-23T12:25:46Z
dc.date.issued2022de
dc.description.abstractSurfactin is described as a powerful biosurfactant and is natively produced by Bacillus subtilis in notable quantities. Among other industrially relevant characteristics, antimicrobial properties have been attributed to surfactin-producing Bacillus isolates. To investigate this property, stress approaches were carried out with biotechnologically established strains of Corynebacterium glutamicum, Bacillus subtilis, Escherichia coli and Pseudomonas putida with the highest possible amounts of surfactin. Contrary to the popular opinion, the highest growth-reducing effects were detectable in B. subtilis and E. coli after surfactin treatment of 100 g/L with 35 and 33%, respectively, while P. putida showed no growth-specific response. In contrast, other antimicrobial biosurfactants, like rhamnolipids and sophorolipids, showed significantly stronger effects on bacterial growth. Since the addition of high amounts of surfactin in defined mineral salt medium reduced the cell growth of B. subtilis by about 40%, the initial stress response at the protein level was analyzed by mass spectrometry, showing induction of stress proteins under control of alternative sigma factors σB and σW as well as the activation of LiaRS two-component system. Overall, although surfactin is associated with antimicrobial properties, relatively low growth-reducing effects could be demonstrated after the surfactin addition, challenging the general claim of the antimicrobial properties of surfactin.en
dc.identifier.swb1799501019
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16792
dc.identifier.urihttps://doi.org/10.3390/microorganisms10040779
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2076-2607de
dc.sourceMicroorganisms; Vol. 10, No. 4 (2022) 779de
dc.subjectBacillus subtilis
dc.subjectSurfactin
dc.subjectSecondary metabolites
dc.subjectBiosurfactants
dc.subjectStress response
dc.subject.ddc660
dc.titleSurfactin shows relatively low antimicrobial activity against Bacillus subtilis and other bacterial model organisms in the absence of synergistic metabolitesen
dc.type.diniArticle
dcterms.bibliographicCitationMicroorganisms, 10 (2022), 4, 779. https://doi.org/10.3390/microorganisms10040779. ISSN: 2076-2607
dcterms.bibliographicCitation.issn2076-2607
dcterms.bibliographicCitation.issue4
dcterms.bibliographicCitation.journaltitleMicroorganisms
dcterms.bibliographicCitation.volume10
local.export.bibtex@article{Lilge2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16792}, doi = {10.3390/microorganisms10040779}, author = {Lilge, Lars and Ersig, Nadine and Hubel, Philipp et al.}, title = {Surfactin Shows Relatively Low Antimicrobial Activity against Bacillus subtilis and Other Bacterial Model Organisms in the Absence of Synergistic Metabolites}, journal = {Microorganisms}, year = {2022}, volume = {10}, number = {4}, }
local.export.bibtexAuthorLilge, Lars and Ersig, Nadine and Hubel, Philipp et al.
local.export.bibtexKeyLilge2022
local.export.bibtexType@article

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