Uptake and time‐dependent subcellular localization of native and micellar curcumin in intestinal cells

dc.contributor.authorFlory, Sandra
dc.contributor.authorBenz, Ann‐Kathrin
dc.contributor.authorFrank, Jan
dc.date.accessioned2024-09-03T14:03:45Z
dc.date.available2024-09-03T14:03:45Z
dc.date.issued2022de
dc.description.abstractUptake into intestinal cells and intracellular distribution into metabolically competent organelles, such as the endoplasmic reticulum, are important processes potentially limiting the bioavailability of xenobiotics. The incorporation of curcumin into polysorbate 80 micelles improves its naturally low oral bioavailability in humans. Here, we investigated uptake and time‐dependent localization of curcumin in intestinal cells when administered as native or micellar formulation. Differentiated Caco‐2 cells were incubated with 200 μmol/L native or micellar curcumin for up to 180 min and cellular uptake was quantified. Intracellular curcumin was detected already after 30 min and did not differ significantly between formulations or over time. Subcellular localization of native and micellar curcumin in Caco‐2 cells was studied by density gradient centrifugation. After 30 min, curcumin from both formulations was mainly associated with mitochondria and lysosomes, after 180 min native curcumin was associated with mitochondria and peroxisomes, micellar curcumin with peroxisomes only. Uptake and localization of native and micellar curcumin in intestinal cells do not differ significantly and consequently do not explain differences in bioavailability in humans. The temporary co‐localization with lysosomes is in agreement with the previously proposed role of endocytosis in cellular uptake of curcumin and warrants further investigation.en
dc.identifier.swb1793490791
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16578
dc.identifier.urihttps://doi.org/10.1002/biof.1828
dc.language.isoengde
dc.rights.licensecc_by-nc-ndde
dc.source1872-8081de
dc.sourceBioFactors; Vol. 48, No. 4 (2022), 897-907de
dc.subjectCaco‐2 cellsen
dc.subjectCurcuminen
dc.subjectLysosomesen
dc.subjectPolysorbate 80 micellesen
dc.subjectSubcellular localizationen
dc.subject.ddc610
dc.titleUptake and time‐dependent subcellular localization of native and micellar curcumin in intestinal cellsen
dc.type.diniArticle
dcterms.bibliographicCitationBioFactors, 48 (2022), 4, 897-907. https://doi.org/10.1002/biof.1828. ISSN: 1872-8081
dcterms.bibliographicCitation.issn1872-8081
dcterms.bibliographicCitation.issue4
dcterms.bibliographicCitation.journaltitleBioFactors
dcterms.bibliographicCitation.volume48
local.export.bibtex@article{Flory2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16578}, doi = {10.1002/biof.1828}, author = {Flory, Sandra and Benz, Ann‐Kathrin and Frank, Jan et al.}, title = {Uptake and time‐dependent subcellular localization of native and micellar curcumin in intestinal cells}, journal = {BioFactors}, year = {2022}, volume = {48}, number = {4}, }
local.export.bibtexAuthorFlory, Sandra and Benz, Ann‐Kathrin and Frank, Jan et al.
local.export.bibtexKeyFlory2022
local.export.bibtexType@techreport

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