Methane reduction potential of brown seaweeds and their influence on nutrient degradation and microbiota composition in a rumen simulation technique

dc.contributor.authorKünzel, Susanne
dc.contributor.authorYergaliyev, Timur
dc.contributor.authorWild, Katharina J.
dc.contributor.authorPhilippi, Hanna
dc.contributor.authorPetursdottir, Asta H.
dc.contributor.authorGunnlaugsdottir, Helga
dc.contributor.authorReynolds, Chris K.
dc.contributor.authorHumphries, David J.
dc.contributor.authorCamarinha-Silva, Amélia
dc.contributor.authorRodehutscord, Markus
dc.date.accessioned2024-10-23T12:25:39Z
dc.date.available2024-10-23T12:25:39Z
dc.date.issued2022de
dc.description.abstractThis study aimed to investigate the effects of two brown Icelandic seaweed samples (Ascophyllum nodosum and Fucus vesiculosus) on in vitro methane production, nutrient degradation, and microbiota composition. A total mixed ration (TMR) was incubated alone as control or together with each seaweed at two inclusion levels (2.5 and 5.0% on a dry matter basis) in a long-term rumen simulation technique (Rusitec) experiment. The incubation period lasted 14 days, with 7 days of adaptation and sampling. The methane concentration of total gas produced was decreased at the 5% inclusion level of A. nodosum and F. vesiculosus by 8.9 and 3.6%, respectively (P < 0.001). The total gas production was reduced by all seaweeds, with a greater reduction for the 5% seaweed inclusion level (P < 0.001). Feed nutrient degradation and the production of volatile fatty acids and ammonia in the effluent were also reduced, mostly with a bigger effect for the 5% inclusion level of both seaweeds, indicating a reduced overall fermentation (all P ≤ 0.001). Microbiota composition was analyzed by sequencing 16S rRNA amplicons from the rumen content of the donor cows, fermenter liquid and effluent at days 7 and 13, and feed residues at day 13. Relative abundances of the most abundant methanogens varied between the rumen fluid used for the start of incubation and the samples taken at day 7, as well as between days 7 and 13 in both fermenter liquid and effluent (P < 0.05). According to the differential abundance analysis with q2-ALDEx2, in effluent and fermenter liquid samples, archaeal and bacterial amplicon sequence variants were separated into two groups (P < 0.05). One was more abundant in samples taken from the treatment without seaweed supplementation, while the other one prevailed in seaweed supplemented treatments. This group also showed a dose-dependent response to seaweed inclusion, with a greater number of differentially abundant members between a 5% inclusion level and unsupplemented samples than between a 2.5% inclusion level and TMR. Although supplementation of both seaweeds at a 5% inclusion level decreased methane concentration in the total gas due to the high iodine content in the seaweeds tested, the application of practical feeding should be done with caution.en
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16753
dc.identifier.urihttps://doi.org/10.3389/fmicb.2022.889618
dc.language.isoengde
dc.rights.licensecc_byde
dc.source1664-302Xde
dc.sourceFrontiers in microbiology; Vol. 13 (2022) 889618de
dc.subjectSeaweed
dc.subjectMacro algae
dc.subjectRumen
dc.subjectMethane
dc.subjectRusitec
dc.subjectMicrobiota
dc.subject16S rRNA gene
dc.subject.ddc630
dc.titleMethane reduction potential of brown seaweeds and their influence on nutrient degradation and microbiota composition in a rumen simulation techniqueen
dc.type.diniArticle
dcterms.bibliographicCitationFrontiers in microbiology, 13 (2022), 889618. https://doi.org/10.3389/fmicb.2022.889618. ISSN: 1664-302X
dcterms.bibliographicCitation.journaltitleFrontiers in microbiology
dcterms.bibliographicCitation.volume13
local.export.bibtex@article{Künzel2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16753}, doi = {10.3389/fmicb.2022.889618}, author = {Künzel, Susanne and Yergaliyev, Timur and Wild, Katharina J. et al.}, title = {Methane Reduction Potential of Brown Seaweeds and Their Influence on Nutrient Degradation and Microbiota Composition in a Rumen Simulation Technique}, journal = {Frontiers in microbiology}, year = {2022}, volume = {13}, }
local.export.bibtexAuthorKünzel, Susanne and Yergaliyev, Timur and Wild, Katharina J. et al.
local.export.bibtexKeyKünzel2022
local.export.bibtexType@article
local.title.fullMethane reduction potential of brown seaweeds and their influence on nutrient degradation and microbiota composition in a rumen simulation technique

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