Effect of a diet rich in galactose or fructose, with or without fructooligosaccharides, on gut microbiota composition in rats

dc.contributor.authorMhd Omar, Nor Adila
dc.contributor.authorDicksved, Johan
dc.contributor.authorKruger, Johanita
dc.contributor.authorZamaratskaia, Galia
dc.contributor.authorMichaëlsson, Karl
dc.contributor.authorWolk, Alicja
dc.contributor.authorFrank, Jan
dc.contributor.authorLandberg, Rikard
dc.date.accessioned2024-09-03T14:03:57Z
dc.date.available2024-09-03T14:03:57Z
dc.date.issued2022de
dc.description.abstractRecent studies suggest that a diet rich in sugars significantly affects the gut microbiota. Adverse metabolic effects of sugars may partly be mediated by alterations of gut microbiota and gut health parameters, but experimental evidence is lacking. Therefore, we investigated the effects of high intake of fructose or galactose, with/without fructooligosaccharides (FOS), on gut microbiota composition in rats and explored the association between gut microbiota and low-grade systemic inflammation. Sprague–Dawley rats (n = 6/group) were fed the following isocaloric diets for 12 weeks (% of the dry weight of the sugars or FOS): (1) starch (control), (2) fructose (50%), (3) galactose (50%), (4) starch+FOS (15%) (FOS control), (5) fructose (50%)+FOS (15%), (6) galactose (50%)+FOS (15%), and (7) starch+olive (negative control). Microbiota composition in the large intestinal content was determined by sequencing amplicons from the 16S rRNA gene; 341F and 805R primers were used to generate amplicons from the V3 and V4 regions. Actinobacteria, Verrucomicrobia, Tenericutes, and Cyanobacteria composition differed between diets. Bifidobacterium was significantly higher in all diet groups where FOS was included. Modest associations between gut microbiota and metabolic factors as well as with gut permeability markers were observed, but no associations between gut microbiota and inflammation markers were observed. We found no coherent effect of galactose or fructose on gut microbiota composition. Added FOS increased Bifidobacterium but did not mitigate potential adverse metabolic effects induced by the sugars. However, gut microbiota composition was associated with several metabolic factors and gut permeability markers which warrant further investigations.en
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16622
dc.identifier.urihttps://doi.org/10.3389/fnut.2022.922336
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2296-861Xde
dc.source; Vol. 9 (2022) 922336de
dc.subjectFructose
dc.subjectGalactose
dc.subjectFructooligosaccharides
dc.subjectGut microbiota
dc.subject16S rRNA
dc.subject.ddc610
dc.titleEffect of a diet rich in galactose or fructose, with or without fructooligosaccharides, on gut microbiota composition in ratsen
dc.type.diniArticle
dcterms.bibliographicCitationFrontiers in nutrition, 9 (2022), 922336. https://doi.org/10.3389/fnut.2022.922336. ISSN: 2296-861X
dcterms.bibliographicCitation.journaltitleFrontiers in nutrition
dcterms.bibliographicCitation.volume9
local.export.bibtex@article{Mhd Omar2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16622}, doi = {10.3389/fnut.2022.922336}, author = {Mhd Omar, Nor Adila and Dicksved, Johan and Kruger, Johanita et al.}, title = {Effect of a diet rich in galactose or fructose, with or without fructooligosaccharides, on gut microbiota composition in rats}, journal = {Frontiers in nutrition}, year = {2022}, volume = {9}, }
local.export.bibtexAuthorMhd Omar, Nor Adila and Dicksved, Johan and Kruger, Johanita et al.
local.export.bibtexKeyMhd Omar2022
local.export.bibtexType@article

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