The sensilla-specific expression and subcellular localization of SNMP1 and SNMP2 reveal novel insights into their roles in the antenna of the desert locust Schistocerca gregaria

dc.contributor.authorCassau, Sina
dc.contributor.authorSander, Doreen
dc.contributor.authorKarcher, Thomas
dc.contributor.authorLaue, Michael
dc.contributor.authorHause, Gerd
dc.contributor.authorBreer, Heinz
dc.contributor.authorKrieger, Jürgen
dc.date.accessioned2024-10-23T12:25:40Z
dc.date.available2024-10-23T12:25:40Z
dc.date.issued2022de
dc.description.abstractSimple Summary: The desert locust, Schistocerca gregaria, can form gigantic swarms of millions of individuals that devastate the vegetation of invaded landscapes. Locust food search, reproduction, and aggregation behaviors are triggered and controlled by complex olfactory signals. Insects detect odorants through different types of olfactory sensilla on the antenna that house olfactory sensory neurons and associated support cells, both of which express the proteins required for olfactory signaling. Among these proteins, two members of the CD36 lipid transporter/receptor family, named sensory neuron membrane proteins 1 and 2 (SNMP1 and SNMP2), are indicated to be of vital importance. Towards a better understanding of the role of the two SNMPs in the olfactory system of S. gregaria, we have analysed their antennal topography and subcellular localization using specific antibodies. The results indicate sensilla type- and cell type-specific distribution patterns of the SNMP proteins. SNMP1 was located in the receptive dendrites of subpopulations of olfactory sensory neurons as well as in the microvilli of associated support cells, suggesting a dual function of this protein, both in olfactory signal detection and in sensillum lymph maintenance, respectively. In contrast, SNMP2 was found solely in support cells and their microvilli membranes, suggesting a role limited to sensillum lymph recovery processes. Abstract: Insect olfactory sensilla house olfactory sensory neurons (OSNs) and supports cells (SCs). The olfactory sensory processes require, besides the odorant receptors (ORs), insect-specific members of the CD36 family, named sensory neuron membrane proteins (SNMPs). While SNMP1 is considered to act as a coreceptor in the OR-mediated detection of pheromones, SNMP2 was found to be expressed in SCs; however, its function is unknown. For the desert locust, Schistocerca gregaria, we previously visualized mRNA for SNMP1 in OSNs and SNMP2 mRNA in cells associated with OSN clusters. Towards an understanding of their functional implication, it is imperative to explore the cellular and the subcellular localization the SNMP proteins. Therefore, we have generated polyclonal antibodies against SNMP1 and SNMP2 and used fluorescence immunohistochemistry (FIHC) to visualize the SNMP proteins. We found SNMP1 in the somata and respective dendrites of all OSNs in trichoid sensilla and in subsets of OSNs in basiconic sensilla. Notably, SNMP1 was also detected in SCs of these sensilla types. In contrast, SNMP2 protein was only visualized in SCs of basiconic and coeloconic sensilla, but not of trichoid sensilla. Exploring the subcellular localization by electron microscopy using anti-SNMP1-ab and anti-SNMP2-ab revealed an immunogold labelling of SC microvilli bordering the sensillum lymph. Together our findings suggest a dual role of SNMP1 in the antenna of S. gregaria, in some OSN subpopulations in odor detection as well as in functions of some SCs, whereas the role of SNMP2 is limited to the functions of support cells.en
dc.identifier.swb1809037395
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16761
dc.identifier.urihttps://doi.org/10.3390/insects13070579
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2075-4450de
dc.sourceInsects; Vol. 13, No. 7 (2022) 579de
dc.subjectOlfaction
dc.subjectLocust
dc.subjectSensilla
dc.subjectOlfactory sensory neuron
dc.subjectSupport cell
dc.subjectOdorant detection
dc.subjectSensory neuron membrane protein
dc.subject.ddc630
dc.titleThe sensilla-specific expression and subcellular localization of SNMP1 and SNMP2 reveal novel insights into their roles in the antenna of the desert locust Schistocerca gregariaen
dc.type.diniArticle
dcterms.bibliographicCitationInsects, 13 (2022), 7, 579. https://doi.org/10.3390/insects13070579. ISSN: 2075-4450
dcterms.bibliographicCitation.issn2075-4450
dcterms.bibliographicCitation.issue7
dcterms.bibliographicCitation.journaltitleInsects
dcterms.bibliographicCitation.volume13
local.export.bibtex@article{Cassau2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16761}, doi = {10.3390/insects13070579}, author = {Cassau, Sina and Sander, Doreen and Karcher, Thomas et al.}, title = {The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria}, journal = {Insects}, year = {2022}, volume = {13}, number = {7}, }
local.export.bibtexAuthorCassau, Sina and Sander, Doreen and Karcher, Thomas et al.
local.export.bibtexKeyCassau2022
local.export.bibtexType@article
local.title.fullThe sensilla-specific expression and subcellular localization of SNMP1 and SNMP2 reveal novel insights into their roles in the antenna of the desert locust Schistocerca gregaria

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