Genomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanism

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dc.contributor.authorSchwedt, Inge
dc.contributor.authorCollignon, Madeline
dc.contributor.authorMittelstädt, Carolin
dc.contributor.authorGiudici, Florian
dc.contributor.authorRapp, Johanna
dc.contributor.authorMeißner, Janek
dc.contributor.authorLink, Hannes
dc.contributor.authorHertel, Robert
dc.contributor.authorCommichau, Fabian M.
dc.date.accessioned2024-07-05T12:49:19Z
dc.date.available2024-07-05T12:49:19Z
dc.date.issued2023
dc.date.updated2024-06-27T03:44:59Z
dc.description.abstractGlyphosate (GS) specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase that converts phosphoenolpyruvate (PEP) and shikimate-3-phosphate to EPSP in the shikimate pathway of bacteria and other organisms. The inhibition of the EPSP synthase depletes the cell of the EPSP-derived aromatic amino acids as well as of folate and quinones. A variety of mechanisms (e.g., EPSP synthase modification) has been described that confer GS resistance to bacteria. Here, we show that the Burkholderia anthina strain DSM 16086 quickly evolves GS resistance by the acquisition of mutations in the ppsR gene. ppsR codes for the pyruvate/ortho-Pi dikinase PpsR that physically interacts and regulates the activity of the PEP synthetase PpsA. The mutational inactivation of ppsR causes an increase in the cellular PEP concentration, thereby abolishing the inhibition of the EPSP synthase by GS that competes with PEP for binding to the enzyme. Since the overexpression of the Escherichia coli ppsA gene in Bacillus subtilis and E. coli did not increase GS resistance in these organisms, the mutational inactivation of the ppsR gene resulting in PpsA overactivity is a GS resistance mechanism that is probably unique to B. anthina.en
dc.identifier10.1111/1758-2229.13184en
dc.identifierEMI413184en
dc.identifier.urihttps://doi.org/10.1111/1758-2229.13184
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/15906
dc.language.isoeng
dc.relation.ispartofEnvironmental Microbiology Reportsen
dc.rights.licensecc_by
dc.subjectGlyphosate resistance
dc.subjectEPSP synthase
dc.subjectShikimate pathway
dc.subjectBurkholderia anthina
dc.subjectppsR gene mutation
dc.subjectPEP synthetase
dc.subjectBacterial resistance mechanisms
dc.subjectMetabolic adaptation
dc.subject.ddc570
dc.titleGenomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanismen
dc.type.diniArticle
dcterms.bibliographicCitationEnvironmental microbiology, 15 (2023), 6, 727-739. https://doi.org/10.1111/1758-2229.13184. ISSN: 1462-2920
dcterms.bibliographicCitation.issn1462-2920
dcterms.bibliographicCitation.issue6
dcterms.bibliographicCitation.journaltitleEnvironmental microbiology
dcterms.bibliographicCitation.originalpublishernameBlackwell
dcterms.bibliographicCitation.originalpublisherplaceOxford [u.a.]
dcterms.bibliographicCitation.pageend739
dcterms.bibliographicCitation.pagestart727
dcterms.bibliographicCitation.volume15
local.export.bibtex@article{Schwedt2023, doi = {10.1111/1758-2229.13184}, author = {Schwedt, Inge and Collignon, Madeline and Mittelstädt, Carolin et al.}, title = {Genomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanism}, journal = {Environmental microbiology}, year = {2023}, volume = {15}, number = {6}, pages = {727--739}, }
local.export.bibtexAuthorSchwedt, Inge and Collignon, Madeline and Mittelstädt, Carolin et al.
local.export.bibtexKeySchwedt2023
local.export.bibtexPages727--739
local.export.bibtexType@article

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