A new version of this entry is available:
Loading...
Article
2022
Serotonin receptor 5-HT2A regulates TrkB receptor function in heteroreceptor complexes
Serotonin receptor 5-HT2A regulates TrkB receptor function in heteroreceptor complexes
Abstract (English)
Serotonin receptor 5-HT2A and tropomyosin receptor kinase B (TrkB) strongly contribute to neuroplasticity regulation and are implicated in numerous neuronal disorders. Here, we demonstrate a physical interaction between 5-HT2A and TrkB in vitro and in vivo using co-immunoprecipitation and biophysical and biochemical approaches. Heterodimerization decreased TrkB autophosphorylation, preventing its activation with agonist 7,8-DHF, even with low 5-HT2A receptor expression. A blockade of 5-HT2A receptor with the preferential antagonist ketanserin prevented the receptor-mediated downregulation of TrkB phosphorylation without restoring the TrkB response to its agonist 7,8-DHF in vitro. In adult mice, intraperitoneal ketanserin injection increased basal TrkB phosphorylation in the frontal cortex and hippocampus, which is in accordance with our findings demonstrating the prevalence of 5-HT2A–TrkB heteroreceptor complexes in these brain regions. An expression analysis revealed strong developmental regulation of 5-HT2A and TrkB expressions in the cortex, hippocampus, and especially the striatum, demonstrating that the balance between TrkB and 5-HT2A may shift in certain brain regions during postnatal development. Our data reveal the functional role of 5-HT2A–TrkB receptor heterodimerization and suggest that the regulated expression of 5-HT2A and TrkB is a molecular mechanism for the brain-region-specific modulation of TrkB functions during development and under pathophysiological conditions.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Notes
Publication license
Publication series
Published in
Cells, 11 (2022), 15, 2384.
https://doi.org/10.3390/cells11152384.
ISSN: 2073-4409
Faculty
Institute
Examination date
Supervisor
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
610 Medicine and health
Collections
Original object
Standardized keywords (GND)
Sustainable Development Goals
BibTeX
@article{Ilchibaeva2022,
url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16594},
doi = {10.3390/cells11152384},
author = {Ilchibaeva, Tatiana and Tsybko, Anton and Zeug, Andre et al.},
title = {Serotonin Receptor 5-HT2A Regulates TrkB Receptor Function in Heteroreceptor Complexes},
journal = {Cells},
year = {2022},
volume = {11},
number = {15},
}