Institut für Phytomedizin

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Cover cropping in integrated weed management
(2018) Sturm, Dominic; Gerhards, Roland
Weed control constitutes a major challenge in the worldwide crop production. Beside chemical and mechanical weed control strategies, cover cropping provides an effective way of biological weed suppression. Five different field experiments were conducted at six locations from 2014-2016 to evaluate the weed control efficacy of different cover crops in mono and mixed cultivation combined with different fertilization strategies and sowing dates. Furthermore weed suppressing effects of cover crop mulches in spring and of living mulches in summer were investigated. Potential effects on sugar beet emergence, quality and quantity were also assessed. In three laboratory and two greenhouse experiments from 2015-2017, the proportional contribution of competitive and biochemical effects on the overall weed suppression and the identification of varying susceptibilities of different weeds against biochemical stresses were at the center of research. In field experiments, the weed suppressive effects of cover crops and living mulches in mono and mixed cultivation were tested. The experiments emphasized the importance of cover crop and living mulch mixtures compared to mono cropping due to a higher flexibility to biotic and abiotic stresses. This was followed by a more constant biomass production and more effective weed suppression. Moreover, the observed weed control was a result of competitive and biochemical effects, induced by cover crops. These were later on analyzed for active weed growth suppressing compounds. Altering cover crop sowing date and fertilization to optimize the weed control resulted in significant changes of cover crop and weed biomass. Early cover crop sowing five or three weeks before winter wheat harvest increased the weed control efficacy in one year, significantly. Due to contrary results over the two experimental years, we suggest that the cover crop biomass and consequently the weed suppressive ability depends on sufficient soil water for rapid cover crop germination and growth. The use of cover crop mulch in sugar beet crops provided a weed suppression of up to 83%. Especially mulch derived from cover crop mixtures reduced the weed density (56%) more effectively compared to mono cultivated cover crops (31%). The inclusion of cover crops, mulches and living mulches can lead to significant herbicide reductions in the main crop. However supplementary mechanical or chemical weed control strategies are still necessary, especially in crops with a low competitive ability like sugar beets. Nevertheless, novel mechanical weed control approaches and adequate herbicide application techniques, as band-spraying, can reduce the herbicide input in the long-term. Germination tests with aqueous cover crop extracts were conducted on weed seeds to evaluate differences in the inhibition of germination and root growth. Furthermore, different sensitivities of the weeds against the different cover crop extracts were revealed. Some cover crops as S. alba, F. esculentum, H. annuus, T. subterraneum and L. usitatissimum showed the most effective weed suppression. Moreover, the weed M. chamomilla showed the highest susceptibility against biochemical stresses in the germination tests. A strong positive correlation between the weed suppressive effects by the extracts and the field weed suppression was found. This indicated that biochemical effects play also an important role on the overall weed suppression in the field. To estimate the proportions of competitive and biochemical effects on the overall weed suppression by cover crops, greenhouse experiments with active carbon supplemented soil were conducted. These experiments revealed that biochemical effects, by the presence of active carbon in the soil, shifted the balance of competition between cover crops and weeds. In the course of the experiments, we also found species-specific effects on the donor as well as on the receiver side. The results of this thesis demonstrate the diverse use of cover crops, their mulches and living mulches in agricultural systems. This work aims on the optimization of biological weed control strategies and indicates approaches for future research. It is for example not yet clear how cover crops suppress specific weeds and if it is possible to design combinations of specific cover crops for the suppression of individual weed communities. Additionally, these results help to reduce long-term herbicide inputs in agricultural systems.
Root exudate fingerprint of Brachiaria humidicola reveals vanillin as a novel and effective nitrification inhibitor
(2023) Egenolf, Konrad; Schöne, Jochen; Conrad, Jürgen; Braunberger, Christina; Beifuß, Uwe; Arango, Jacobo; Rasche, Frank
Introduction: Biological Nitrification Inhibition (BNI) is defined as the plant-mediated control of soil nitrification via the release of nitrification inhibitors. BNI of Brachiaria humidicola (syn. Urochloa humidicola) has been mainly attributed to root-exuded fusicoccane-type diterpenes, e.g., 3-epi-brachialactone. We hypothesized, however, that BNI of B. humidicola is caused by an assemblage of bioactive secondary metabolites. Methods: B. humidicola root exudates were collected hydroponically, and metabolites were isolated by semi-preparative HPLC. Chemical structures were elucidated by HRMS as well as 1D and 2D NMR spectroscopy. Nitrification inhibiting potential of isolated metabolites was evaluated by a Nitrosomonas europaea based bioassay. Results and discussion: Besides previously described brachialactone isomers and derivatives, five phenol and cinnamic acid derivatives were identified in the root exudates of B. humidicola: 2-hydroxy-3-(hydroxymethyl)benzaldehyde, vanillin, umbelliferone and both trans- and cis-2,6-dimethoxycinnamic acid. Notably, vanillin revealed a substantially higher nitrification inhibiting activity than 3-epi-brachialactone (ED50 ∼ 12.5 μg·ml−1, ED80 ∼ 20 μg·ml−1), identifying this phenolic aldehyde as novel nitrification inhibitor (NI). Furthermore, vanillin exudation rates were in the same range as 3-epi-brachialactone (1–4 μg·h−1·g−1 root DM), suggesting a substantial contribution to the overall inhibitory activity of B. humidicola root exudates. In relation to the verification of the encountered effects within soils and considering the exclusion of any detrimental impact on the soil microbiome, the biosynthetic pathway of vanillin via the precursor phenylalanine and the intermediates p-coumaric acid/ferulic acid (precursors of further phenolic NI) might constitute a promising BNI breeding target. This applies not only to Brachiaria spp., but also to crops in general, owing to the highly conserved nature of these metabolites.

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