Browsing by Subject "Grassland"

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Approaches to improve the implementation and expansion of Miscanthus production
(2016) Xue, Shuai; Lewandowski, Iris
Several species within the miscanthus genus (Miscanthus spp.) are characterized by high biomass yields and low production input requirements. This raised increasing interests in their applications for bioenergy. However, to date, only small areas of Miscanthus × giganteus (approximately 40,000 ha) are commercially grown and used for generating electricity and heat in Europe, where miscanthus has been developed as bioenergy crop for more than decade. Reviewing state-of-the-art revealed four main factors limiting the implementation of miscanthus production. These are inefficient and expensive propagation techniques, land use dilemma (i.e. lack of land available for growing miscanthus), lack of varieties/genotypes adapted to various and especially to stressful environmental conditions and lack of efficient agronomic practices for miscanthus establishment. Against these limiting factors, this thesis aims to (1) evaluate the different propagation systems with regard to technologies and costs, and improve the preferred rhizome propagation techniques; (2) address the land use dilemma through exploring marginal land (i.e. non-arable land with ability to grow plants with tolerance to environmental stresses) for miscathus production; (3) and screen optimal genotypes and effective practices for establishing and managing miscanthus on marginal land in a case study on grassland. To achieve the first objective, a review, our own field trials and farmer surveys were performed. Direct seed sowing was found to be the cheapest propagation method (1,508.5 € ha-1 overall establishment costs) and micro-propagation the most expensive (6,320.8 € ha-1). Direct rhizome planting is the farmers’ most preferred and most applied establishment method and has moderate establishment cost of 1,904-3,375.7 € ha-1. However, it goes along with the lowest propagation efficiency (1:10) and consequently restricts the availability of propagation material for large-scale plantations. However, the multiplication ratio can be increased by reducing the rhizome size. Field trial results showed that 6-cm length is close to the minimum size of rhizome that can germinate after directly planting into field. Compared to the traditionally used macro-rhizome, the multiplication ratio of the improved rhizome propagation (using 6-cm rhizomes) is tripled. In addition, the multiplication ratio can also be increased by transplanting rhizome- or stem-derived plantlets. However, due to higher labour and energy inputs required for the pre-growing of plantlets, their establishment cost reduction potential is limited, with estimated costs of 4,240.8-4,400.8 € ha-1. Direct seed sowing as the cheapest method is presently only possible for Miscanthus sinensis and not yet practical under German conditions. In addition, the seed-setting rate of M. sinensis is very low (0.0-28.7%) under the climatic conditions of south-west Germany, making commercial seeds production difficult. For all the propagation methods considered, more research efforts are still required to reduce the material production costs and simultaneously increase the multiplication ratio. For the second objective, the production potential of miscanthus on marginal land in China was assessed. Because China has limited agricultural land resources and its non-food bioenergy policy (it is only allowed to grow energy crops on marginal land) is adamant, there is a desideration for exploiting its marginal land potential. In this study, Geographic Information System (GIS) techniques, model simulation were adopted to identify the productive marginal areas for miscanthus and to estimate their biomass and bioenergy production potentials. The results show that in China there are large marginal land areas of 17,163.54 × 104 ha available for growing miscanthus. However, due to limitation by low winter temperatures and low precipitation levels in some areas, the total marginal area suitable for growing miscanthus is only 769.37 × 104 ha. The Monteith radiation yield model was used to determine the potential miscanthus yield in Chinese climatic conditions. The simulation gave the actual harvestable yield levels on arable land of 18.1-44.2 odt ha-1 yr-1. Taking the environmental stresses of marginal conditions into account an achievable miscanthus yield potential on marginal land of 2.1-32.4 odt ha-1 yr-1 was calculated (varying between different marginal land types). Based on these achievable yield levels, the total miscanthus production potential on the entire suitable marginal land areas is 13,521.7 × 104 odt yr-1; the corresponding bio-electricity generation and total greenhouse gas saving potentials are 183.9 TW h yr-1 and 21,242.4 × 104 t CO2 eq. yr-1, respectively. The spatial distribution of the suitable marginal areas shows that they are mainly concentrated in the central part of Northeast China and the Loess Plateau. Both regions are recommended as priority development zones for the Chinese miscanthus-based bioenergy industry. However, implementation of this huge marginal land potential is currently constrained by many barriers, e.g. concerns on potential ecological effects, competition for marginal land from other uses, lack of high yield varieties in marginal conditions. Lack of varieties with suitability to marginal conditions and efficient agronomic practices for the establishment on marginal land are the main barriers that limit using marginal land for miscanthus production. Therefore, stress tolerant varieties need to be selected and methods of effective establishment of miscanthus on marginal land need to be developed. Worldwide, grassland is the most important marginal land type because it has the largest terrestrial area and mild environmental stresses for growing energy crops (including miscanthus). However, it is undesirable or even legally prohibited to convert grassland into bioenergy cropland to avoid biodiversity loss and soil carbon being reduced by tilling practices. Hence, no-till establishment practices for miscanthus establishment and maintenance on grassland are investigated here under the third objectives. Our study demonstrates that miscanthus can be successfully cultivated on both good (nutrient-rich) and marginal (nutrient-poor) grassland using the proposed agronomic practices and an increased grassland productivity may be achieved through the establishment of suitable miscanthus genotypes. The recommended agronomic practices are summarized as following. Miscanthus genotypes with tall, thick shoots perform better than those with short, thin shoots. Better establishment is achieved when rhizome-derived plantlets are transplanted into pre-disturbed grassland. The grassland pre-disturbance of low vegetation cutting (5 cm) and herbicide spraying in narrow stripes is recommended for its beneficial effect on miscanthus establishment without significant negative effects on grassland productivity. Two harvests, one in late spring and one in late autumn, are optimal to achieve a high grassland yield. In this thesis, the limitation of the inefficient propagation technique was mitigated through minimizing the rhizome size and exploring the seeds propagation potential. The land-use dilemma was alleviated by exploring the marginal land production potential. Additionally, constrains of lack of genotypes and agronomic practices for the miscanthus establishment on marginal land were improved by field trials on grassland (the most important marginal land type with a huge potential).These results can improve the implementation and expansion of miscanthus production. However, in addition to constrains improved in this thesis, the miscanthus production is currently constrained by many other technical, economic and financial, social and political, environmental issues. It is unlikely that the implementation and expansion will achieve without mitigating these constrains. Further research and support should address these barriers in an integrate manner.
Gesamtbetriebliche Nachhaltigkeitsanalyse von Grünlandsystemen in der Milchviehhaltung in benachteiligten Regionen Südwestdeutschlands
(2020) Dentler, Juliane; Elsäßer, Martin
Agriculture is facing major global and regional challenges. The reduction of greenhouse gas and ammonia emissions, the lowering of nutrient discharges, serious losses of biodiversity and food security require urgent action, to determine sustainable structures for the production of agricultural products in general and animal products in particular and to develop solution strategies for the efficient use of resources. A central element of sustainable development is the equal consideration of the three pillars of sustainability - ecology, economy and social aspects. Against this background, the research project A sustainability comparison of grassland-based feeding systems for milk production in disadvantaged regions of south-west Germany was conceived. The aim of the research study was a system comparison at farm level, which not only shows the ecological potential but also the economic and social factors involved in order to improve the understanding of the sustainability of agricultural farm systems and thus contribute to an optimised use of regional resources. For this purpose, grassland-based, concentrate feed-reduced systems of milk production in disadvantaged regions and successful milk systems of year-round barn management with high individual animal performance in favoured areas of southwest Germany were compared. In addition, the potential of using the regional dual-purpose breed Vorderwald for grassland-based, concentrate feed-reduced milk systems was examined. In the present study, the economically most successful farms of the grassland-based sample displayed a high economic efficiency in the calculated management income compared to conventional dairy farms. Nevertheless, it became clear, that economically sustainable milk production is not given at all locations. In principle, organic dairy farms in grassland regions can profit economically from high prices for organic milk together with subsidies from agri-environmental measures combined with a high use of pasture grass and low external costs (concentrated feed, external labour). This was also evident with regard to the social parameters, especially in the areas of stress, leisure time and appreciation by the population and by colleagues, a high degree of social sustainability was demonstrated. With regard to the efficiency parameters, the farms of the grassland-based sample performed significantly better than the intensive dairy farms, especially with regard to the net food conversion efficiency, with an average of 295 % related to gross energy, and the nutrient efficiency, with a 10 % higher use efficiency of nitrogen and a 55 % higher use efficiency of phosphorus. The latter were characterised by an average nitrogen surplus of 150 kg per ha. By taking into account the CO2-storage in conjunction with the integration of ecosystem services in the allocation of emissions, it was also possible to demonstrate a lower carbon footprint for grassland-based milk production in disadvantaged regions of southwest Germany. Furthermore, it could be shown that the same feeding system cannot be applied equally beneficially for all breeds and that Vorderwald cattle are better adapted to a system with full pasture, low concentrated feed input and seasonal calving compared to Holstein cattle. Complex agricultural processes ultimately mean that, depending on production methods and consumption, almost identical products have very different effects on the environment, climate, income, social aspects and health. The present study shows that milk production with a high proportion of permanent grassland, adapted animal genetics and a very low dependence on external production factors such as energy- and protein-rich fodder as well as chemical synthetic fertilizers and pesticides, has significant advantages over milk production in intensive systems and can be regarded as eco-nomically, ecologically and socially sustainable. A consistent implementation of this strategy towards systems in which smaller, more robust animals of a dual-purpose breed produce milk and meat almost exclusively on the basis of their natural feed base with a high efficiency per hectare could therefore indicate a direction towards more sustainable milk production, livestock farming and agriculture in general. In this context, organic farming is seen as particularly resource-saving and environmentally compatible and is discussed as a concept to improve sustainability in agriculture. For sustainable development and defusing conflicts of objectives, however, an overall perspective must be adopted; looking at individual aspects of production is not sufficient. In addition to adjustments in agriculture, far-reaching changes in consumer behaviour and food waste are necessary. This will require considerable political will and cooperation at farm and society level
Influence of land use on abundance, function and spatial distribution of N-cycling microorganisms in grassland soils
(2015) Keil, Daniel; Kandeler, Ellen
This thesis focuses on the influence of land use on the abundance, function and spatial distribution of N-cycling microorganisms in grassland soils, but also on soil biogeochemical properties, as well as on enzyme activities involved in the carbon-, nitrogen-, and phosphorous cycle. The objective of this thesis was tackled in three studies. All study sites that were investigated as part of this thesis were preselected and assigned according to study region and land use within the framework of the “Exploratories for Functional Biodiversity Research – The Biodiversity Exploratories” of the Deutsche Forschungsgemeinschaft priority program 1374. The first study addressed the question whether land-use intensity influences soil biogeochemical properties, as well as the abundance and spatial distributions of ammonia-oxidizing and denitrifying microorganisms in grasslands of the Schwäbische Alb. To this end, a geostatistical approach on replicated grassland sites (10 m × 10 m), belonging to either unfertilized pastures (n = 3) or fertilized mown meadows (n = 3), representing low and high land-use intensity, was applied. Results of this study revealed that land-use intensity changed spatial patterns of both soil biogeochemical properties and N-cycling microorganisms at the plot scale. For soil biogeochemical properties, spatial heterogeneity decreased with higher land-use intensity, but increased for ammonia oxidizers and nirS-type denitrifiers. This suggests that other factors, both biotic and abiotic than those measured, are driving the spatial distribution of these microorganisms at the plot scale. Furterhmore, the geostatistical analysis indicated spatial coexistence for ammonia oxidizers (amoA ammonia-oxidizing archaea and amoA ammonia-oxidizing bacteria) and nitrate reducers (napA and narG), but niche partitioning between nirK- and nirS-type denitrifiers. The second study aimed at whether land-use intensity contributes to spatial variation in microbial abundance and function in grassland ecosystems of the Schwäbische Alb assigned to either low (unfertilized pastures, n = 3), intermediate (fertilized mown pastures, n = 3), or high (fertilized mown meadows, n = 3) land-use intensity. Plot-scale (10 m × 10 m) spatial heterogeneity and autocorrelation of soil biogeochemical properties, microbial biomass and enzymes involved in C, N, and P cycle were investigated using a geostatistical approach. Geostatistics revealed spatial autocorrelations (p-Range) of chemical soil properties within the maximum sampling distance of the investigated plots, while greater variations of p-Ranges of soil microbiological properties indicated spatial heterogeneity at multiple scales. An expected decrease in small-scale spatial heterogeneity in high land-use intensity could not be confirmed for microbiological soil properties. Finding smaller spatial autocorrelations for most of the investigated properties indicated increased habitat heterogeneity at smaller scales under high land-use intensity. In the third study, the effects of warming and drought on the abundance of denitrifier marker genes, the potential denitrification activity and the N2O emission potential from grassland ecosystems located in the Schwäbische Alb, the Hainich, and the Schorfheide region were investigated. Land use was defined individually for each grassland site by a land-use index that integrated mowing, grazing and fertilization at the sites over the last three years before sampling of the soil. It was tested if the microbial community response to warming and drought depended on more static site properties (soil organic carbon, water holding capacity, pH) in interaction with land use, the study region and the climate change treatment. It was further tested to which extent the N2O emission potential was influenced by more dynamic properties, e.g. the actual water content, the availability of organic carbon and nitrate, or the size of the denitrifier community. Warming effects in enhanced the potential denitrification of denitrifying microorganisms. While differences among the study regions were mainly related to soil chemical and physical properties, the land-use index was a stronger driver for potential denitrification, and grasslands with higher land use also had greater potentials for N2O emissions. The total bacterial community did not respond to experimental treatments, displaying resilience to minor and short-term effects of climate change. In contrast, the denitrifier community tended to be influenced by the experimental treatments and particularly the nosZ abundance was influenced by drought. The results indicate that warming and drought affected the denitrifying communities and the potential denitrification, but these effects are overruled by study region and site-specific land-use index. This thesis gives novel insights into the performance of N-cycling microorganisms in grassland ecosystems. The spatial distribution of soil biogeochemical properties is strongly dependent on land-use intensity, as in return is the spatial distribution of nitrifying and denitrifying microorganisms and the ecosystem services they perform. Yet, future work will be necessary to fully understand the interrelating factors and seasonal variability, which influence the ecosystem functioning and ecosystem services that are provided by N-cycling soil microorganisms at multiple scales.
Linking microbial abundance and function to understand nitrogen cycling in grassland soils
(2017) Regan, Kathleen Marie; Kandeler, Ellen
This thesis characterized spatial and temporal relationships of the soil microbial community, the nitrogen cycling microbial community, and a subset of the nitrogen cycling community with soil abiotic properties and plant growth stages in an unfertilized temperate grassland. Unfertilized perennial grasslands depend solely on soil-available nitrogen and in these environments nitrogen cycling is considered to be both highly efficient and tightly coupled to plant growth. Unfertilized perennial grasslands with high plant diversity, such as ours, have also been shown to have higher soil organic carbon, total nitrogen, and microbial carbon; greater food web complexity; and more complex biological communities than more intensively managed grasslands or croplands. This made the choice of study plot especially well-suited for characterizing the relationships we sought to identify, and made it possible to detect spatial and temporal patterns at a scale that has heretofore been under-examined. The first study used a combination of abiotic, plant functional group, and PLFA measurements together with spatial statistics to interpret spatial and temporal changes in the microbial community over a season. We found that its overall structure was strongly related to the abiotic environment throughout the sampling period. The strength of that relationship varied, however, indicating that it was not constant over time and that other factors also influenced microbial community composition. PLFA analysis combined with principal components analysis made it possible to discern changes in abundances and spatial distributions among Gram-positive and Gram-negative bacteria as well as saprotrophic fungi. Modeled variograms and kriged maps of the changes in distributions of exemplary lipids of both bacterial groups also showed distinct differences in their distributions on the plot, especially at stages of most rapid plant growth. Although environmental properties were identified as the main structuring agents of the microbial community, components of those environmental properties varied over the season, suggesting that plant growth stage had an indirect influence, providing evidence of the complexity and dynamic nature of the microbial community in a grassland soil. The second study took the same analytical approach, this time applying it to abundances of key members of the soil nitrogen cycling community. Marker genes for total archaea and bacteria, nitrogen fixing bacteria, ammonia oxidizing archaea and bacteria, and denitrifying bacteria were quantified by qPCR. Potential nitrification activity and denitrifying enzyme activity were also determined. We found clear seasonal changes in the patterns of abundance of the measured genes and could associate these with changes in substrate availability related to plant growth stages. Most strikingly, we saw that small and ephemeral changes in soil environmental conditions resulted in changes in these microbial communities, while at the same time, process rates of their respective potential enzyme activities remained relatively stable. This suggests both short term niche-partitioning and functional redundancy within the nitrogen cycling microbial community. The seasonal changes in abundances we observed also provided additional evidence of a dynamic relationship between microorganisms and plants, an important mechanism controlling ecosystem nitrogen cycling. The third study determined spatial and temporal interactions between AOA, AOB and NOB. These steps are related in both space and time, as the ammonia-oxidizers provide the necessary substrate for nitrite-oxidizers. Using a combination of spatial statistics and phylogenetic analysis, our data indicated seasonally varying patterns of niche differentiation between the two bacterial groups, Nitrospira and Nitrobacter in April, but more homogeneous patterns by August which may have been due to different strategies for adapting to changes in substrate concentrations resulting from competition with plants. We then asked a further question: was the microbial structure at sampling sites with high NS gene abundances fundamentally different from those with low NS gene abundances? Using a phylogenetic approach, the operational taxonomic unit composition of NS was analyzed. Community composition did not change over the first half of the season, but by the second half, the relative proportion of a particular OTU had increased significantly. This suggested an intraspecific competition within the NS and the possible importance of OTU 03 in nitrite oxidation at a specific period of time. Observed positive correlations between AOA and Nitrospira further suggested that in this unfertilized grassland plot, the nitrification process may be predominantly performed by these groups, but is restricted to a limited timeframe.
Untersuchungen an Werkzeugen für die Direkteinbringung von Flüssigmist in Grünland
(1993) Walter, Andreas; Kutzbach, Heinz Dieter
Die unangenehmsten Geruchsbelästigungen, die von Tierhaltungsbetrieben ausgehen, entstehen bei der Flüssigmistausbringung. Darüber hinaus finden Ammoniakemissionen ein wachsendes Interesse, da sie an der Ausbildung komplexer Umweltschäden beteiligt sind. Auf Grünland ist der Emissionsdruck nach der Flüssigmistausbringung besonders hoch, da die Gülle auf einer großen Pflanzenoberfläche verteilt wird und nicht sofort mit dem Boden in Kontakt kommt. Eine sofortige Einarbeitung des ausgebrachten Flüssigmistes in den Boden kann die genannten Probleme teilweise entschärfen. In der vorliegenden Arbeit werden hierzu verschiedene Verfahren auf Grünland untersucht.
Untersuchungen zur Reduzierung der Ammoniakemissionen nach der Ausbringung von Rinderflüssigmist auf Grünland
(2000) Reitz, Petra; Kutzbach, Heinz Dieter
Ammoniakemissionen stammen zum Großteil aus der landwirtschaftlichen Tierhaltung etwa die Hälfte wird durch die Ausbringung von Flüssigmist verursacht. Unter ungünstigen Bedingungen kann der mit dem Flüssigmist ausgebrachte Ammonium-Stickstoff fast vollständig als NH3-Gas in die Atmosphäre emittieren. Negative Folgewirkungen sind die Verringerung des Düngewertes, eine ungesicherte Düngewirkung sowie nachhaltige Umweltschädigungen. Ziel der Untersuchungen war die Bestimmung der Einflussfaktoren auf die NH3-N-Emissionen nach der Ausbringung von Rinderflüssigmist auf Grünland. Es wurden verschiedene Witterungsbedingungen und verfahrenstechnische Maßnahmen wie Ausbringverfahren, Ausbringmenge und veränderte Zusammensetzung des Flüssigmistes untersucht.