A new version of this entry is available:
Loading...
Article
2023
Dynamic development of viral and bacterial diversity during grass silage preservation
Dynamic development of viral and bacterial diversity during grass silage preservation
Abstract (English)
Ensilaging is one of the most common feed preservation processes using lactic acid bacteria to stabilize feed and save feed quality. The silage bacterial community is well known but the role of the virome and its relationship with the bacterial community is scarce. In the present study, metagenomics and amplicon sequencing were used to describe the composition of the bacterial and viral community during a 40-day grass silage preservation. During the first two days, we observed a rapid decrease in the pH and a shift in the bacterial and viral composition. The diversity of the dominant virus operational taxonomic units (vOTUs) decreased throughout the preservation. The changes in the bacterial community resembled the predicted putative host of the recovered vOTUs during each sampling time. Only 10% of the total recovered vOTUs clustered with a reference genome. Different antiviral defense mechanisms were found across the recovered metagenome-assembled genomes (MAGs); however, only a history of bacteriophage infection with Lentilactobacillus and Levilactobacillus was observed. In addition, vOTUs harbored potential auxiliary metabolic genes related to carbohydrate metabolism, organic nitrogen, stress tolerance, and transport. Our data suggest that vOTUs are enriched during grass silage preservation, and they could have a role in the establishment of the bacterial community.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Notes
Publication license
Publication series
Published in
Viruses, 15 (2023), 4, 951.
https://doi.org/10.3390/v15040951.
ISSN: 1999-4915
Faculty
Institute
Examination date
Supervisor
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
630 Agriculture
Collections
Original object
Free keywords
Standardized keywords (GND)
BibTeX
@article{Sáenz2023,
url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16328},
doi = {10.3390/v15040951},
author = {Sáenz, Johan S. and Rios-Galicia, Bibiana and Rehkugler, Bianca et al.},
title = {Dynamic Development of Viral and Bacterial Diversity during Grass Silage Preservation},
journal = {Viruses},
year = {2023},
volume = {15},
number = {4},
}