A new version of this entry is available:
Loading...
Article
2021
GC/EI-MS method for the determination of phytosterols in vegetable oils
GC/EI-MS method for the determination of phytosterols in vegetable oils
Abstract (English)
Sterols are a highly complex group of lipophilic compounds present in the unsaponifiable matter of virtually all living organisms. In this study, we developed a novel gas chromatography with mass spectrometry selected ion monitoring (GC/MS-SIM) method for the comprehensive analysis of sterols after saponification and silylation. A new referencing system was introduced by means of a series of saturated fatty acid pyrrolidides (FAPs) as internal standards. Linked with retention time locking (RTL), the resulting FAP retention indices (RIFAP) of the sterols could be determined with high precision. The GC/MS-SIM method was based on the parallel measurement of 17 SIM ions in four time windows. This set included eight molecular ions and seven diagnostic fragment ions of silylated sterols as well as two abundant ions of FAPs. Altogether, twenty molecular ions of C27- to C31-sterols with 0–3 double bonds were included in the final method. Screening of four common vegetable oils (sunflower oil, hemp oil, rapeseed oil, and corn oil) enabled the detection of 30 different sterols and triterpenes most of which could be identified.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Other version
Notes
Publication license
Publication series
Published in
Analytical and bioanalytical chemistry, 414 (2021), 2, 1061-1071.
https://doi.org/10.1007/s00216-021-03730-9.
ISSN: 1618-2650
Other version
Faculty
Institute
Examination date
Supervisor
Cite this publication
Schlag, S., Huang, Y., & Vetter, W. (2021). GC/EI-MS method for the determination of phytosterols in vegetable oils. Analytical and bioanalytical chemistry, 414(2). https://doi.org/10.1007/s00216-021-03730-9
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
660 Chemical engineerin
Collections
Original object
University bibliography
Free keywords
Standardized keywords (GND)
Sustainable Development Goals
BibTeX
@article{Schlag2021,
url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16459},
doi = {10.1007/s00216-021-03730-9},
author = {Schlag, Sarah and Huang, Yining and Vetter, Walter et al.},
title = {GC/EI-MS method for the determination of phytosterols in vegetable oils},
journal = {Analytical and bioanalytical chemistry},
year = {2021},
volume = {414},
number = {2},
}