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Bioprinting of 3D adipose tissue models using a GelMA-bioink with human mature adipocytes or human adipose-derived stem cells

dc.contributor.authorAlbrecht, Franziska Brigitte
dc.contributor.authorSchmidt, Freia F.
dc.contributor.authorVolz, Ann-Cathrin
dc.contributor.authorKluger, Petra J.
dc.date.accessioned2024-09-03T14:03:42Z
dc.date.available2024-09-03T14:03:42Z
dc.date.issued2022de
dc.description.abstractAdipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as an adipose tissue control to evaluate in vitro generated adipose tissue models. During culture, lobule exhibited a stable weight, lactate dehydrogenase, and glycerol release over 15 days. For building up in vitro adipose tissue models, we adapted the biomaterial gelatin methacryloyl (GelMA) composition and handling to homogeneously mix and bioprint human primary mature adipocytes (MA) and adipose-derived stem cells (ASCs), respectively. Accelerated cooling of the bioink turned out to be essential for the homogeneous distribution of lipid-filled MAs in the hydrogel. Last, we compared manual and bioprinted GelMA hydrogels with MA or ASCs and the explanted lobules to evaluate the impact of the printing process and rate the models concerning the physiological reference. The viability analyses demonstrated no significant difference between the groups due to additive manufacturing. The staining of intracellular lipids and perilipin A suggest that GelMA is well suited for ASCs and MA. Therefore, we successfully constructed physiological in vitro models by bioprinting MA-containing GelMA bioinks.en
dc.identifier.swb1822827655
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16560
dc.identifier.urihttps://doi.org/10.3390/gels8100611
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2310-2861de
dc.sourceGels; Vol. 8, No. 10 (2022) 611de
dc.subjectAdipose tissue
dc.subject3D bioprinting
dc.subjectHydrogels
dc.subjectPrimary human adipose-derived stem cells
dc.subjectPrimary human mature adipocytes
dc.subjectGelatin methacryloyl
dc.subject.ddc610
dc.titleBioprinting of 3D adipose tissue models using a GelMA-bioink with human mature adipocytes or human adipose-derived stem cellsen
dc.type.diniArticle
dcterms.bibliographicCitationGels, 8 (2022), 10, 611. https://doi.org/10.3390/gels8100611. ISSN: 2310-2861
dcterms.bibliographicCitation.issn2310-2861
dcterms.bibliographicCitation.issue10
dcterms.bibliographicCitation.journaltitleGels
dcterms.bibliographicCitation.volume8
local.export.bibtex@article{Albrecht2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16560}, doi = {10.3390/gels8100611}, author = {Albrecht, Franziska B. and Schmidt, Freia F. and Volz, Ann-Cathrin et al.}, title = {Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells}, journal = {Gels}, year = {2022}, volume = {8}, number = {10}, }
local.export.bibtexAuthorAlbrecht, Franziska B. and Schmidt, Freia F. and Volz, Ann-Cathrin et al.
local.export.bibtexKeyAlbrecht2022
local.export.bibtexType@article

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