Different quality classes of decomposing plant residues influence dissolved organic matter stoichiometry which results in different soil microbial processing

Abstract

The influence of the quantities and ratios of dissolved organic carbon (DOC) and dissolved nitrogen (DN) generated by different chemical quality classes of organic residues on soil microbial processes in the decomposition process is not well understood. If the DOC-to-DN ratio (hereafter, ratio) of the substrate is close to that of the microbial C-to-N ratio, then the DOC-and-DN stoichiometry of the substrate is balanced, resulting in enhanced microbial processing, i.e., carbon use efficiency (CUE). Uncertainty exists about the influence of DN and the DOC-to-DN ratio on CUE, particularly in high-quality class (high nitrogen) residue-treated soils. A long-term field experiment was used to explore the effect of the annual application of residues of different quality classes on decomposition processes, focusing on the effects of DOC, DN, and the ratio on the microbial metabolic quotient (qCO2), which is the inverse of CUE. DOC and DN were extracted from soils during the 13th year of the experiment. Soils treated with high-quality class groundnut residue (high-nitrogen) had higher DN (5.4 ± 2.6 mg N kg−1) and a lower ratio (6.8 ± 2.6) than those treated with medium-quality (medium-nitrogen) tamarind (3.0 ± 0.6 and 10.7 ± 2.2, respectively). The positive influence of DN on qCO2 (R2 = 0.49 *) in groundnut-treated soil suggested that the high bioavailability of DN reduced CUE due to imbalanced DOC-and-DN stoichiometry. This contradicted earlier published findings on high-nitrogen residues which had balanced DOC-and-DN stoichiometry. The positive influence of the ratio on qCO2 under the tamarind-treated soil (R2 = 0.60 *) indicated that its balanced DOC-and-DN stoichiometry enhanced CUE. High-quality class organic residues can result in either higher or lower CUE than their lower-quality class counterparts depending on whether the resulting DOC-and-DN stoichiometry is balanced or imbalanced.