(2025) Hiller, Eric; Hermann, Alexander; Lilge, Lars
Bioproduction of bioactive compounds on a large-scale poses challenges that require molecular strain engineering. By integrating the principle of genetic code expansion (GCE), the biosynthesis of the surfactin biosurfactant has been controlled in the production host Bacillus subtilis depending on the supply of a defined non-canonical amino acid. The regulation of this molecular process control has been transferred to high-cell-density fed-batch bioreactor procedures, allowing an initial upscaling.
Energy-rich foods lead to an increase of overweight people in our societies. Mainly the fat and sugar content in foods is responsible for this progress. However, both components are very important for the taste and acceptance of foods. Thus, the abundant available lactose will be enzymatically modified by using the enzymes β-galactosidase, L-arabinose isomerase and glucose isomerase in order to generate a new natural sugar syrup. The new sugar mixture will be much sweeter than lactose.
