Institut für Agrartechnik

Permanent URI for this collectionhttps://hohpublica.uni-hohenheim.de/handle/123456789/19

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Challenges of green production of 2,5‐furandicarboxylic acid from bio‐derived 5‐hydroxymethylfurfural: Overcoming deactivation by concomitant amino acids
(2022) Neukum, Dominik; Baumgarten, Lorena; Wüst, Dominik; Sarma, Bidyut Bikash; Saraçi, Erisa; Kruse, Andrea; Grunwaldt, Jan‐Dierk
The oxidation of 5‐hydroxymethylfurfural (HMF) to 2,5‐furandicarboxylic acid (FDCA) is highly attractive as FDCA is considered as substitute for the petrochemically derived terephthalic acid. There are only few reports on the direct use of unrefined HMF solutions from biomass resources and the influence of remaining constituents on the catalytic processes. In this work, the oxidation of HMF in a solution as obtained from hydrolysis and dehydration of saccharides in chicory roots was investigated without intermediate purification steps. The amount of base added to the solution was critical to increase the FDCA yield. Catalyst deactivation occurred and was attributed to poisoning by amino acids from the bio‐source. A strong influence of amino acids on the catalytic activity was found for all supported Au, Pt, Pd, and Ru catalysts. A supported AuPd(2 : 1)/C alloy catalyst exhibited both superior catalytic activity and higher stability against deactivation by the critical amino acids.
Combined butyric acid and methane production from grass silage in a novel green biorefinery concept
(2022) Steinbrenner, Jörg; Müller, Joachim; Oechsner, Hans
In a Green Biorefinery, grass silage can be a source for lactic acid, proteins, amino acids and fibres. Processing residues can be used for anaerobic digestion and methane production. But by changing the ensiling conditions, butyric acid fermentation can be achieved. That makes grass silage also a potential substrate for a combined butyric acid and methane production. The objective of this study was to determine the potential of butyric acid production at different ensiling conditions applied to grass and measuring the methane yield potential of solid residues after a separation step. The highest butyric acid concentration in the produced press juice was 20.1 ± 4.5 g kg⁻¹ and was achieved by carbonated lime addition and a reduced dry matter content after 90 days at mesophilic storage conditions. This resulted in a theoretical butyric acid yield of 332 kg ha⁻¹ a⁻¹. For the fibrous leftover press cake, a theoretical methane production potential of 2778 m3CH4 ha⁻¹ a⁻¹ was reached. The results show that theoretically a combined production of butyric acid and methane can be realised in a Green Biorefinery concept.
Effects of harvest date and ensiling additives on the optimized ensiling of Silphium perfoliatum to prevent faulty fermentation
(2024) Baumgart, Marian; Hülsemann, Benedikt; Sailer, Gregor; Oechsner, Hans; Müller, Joachim; Hu, Wei; Zhou, Zhiguo; Zhao, Wenqing
Silphium perfoliatum , an energy crop with a high fiber content but low concentrations of fermentable carbohydrates, presents challenges for complete fermentation in biogas production. To overcome this, a bioeconomic approach proposes the use of the fibers for paper and board production, which requires high-quality silage with minimal butyric acid, which affects the marketability of the fibers. This study aims to optimize the silaging process of Silphium perfoliatum by investigating the effects of harvest date, bacterial cultures and additives on fermentation results. Laboratory experiments were conducted to evaluate the effect of three harvest dates on fermentation acid composition, with a focus on increasing lactic acid production to inhibit butyric acid formation. Results indicate that an early harvest date (early September) is critical for achieving stable fermentation and minimizing ensiling losses. The addition of sugar-rich additives, such as syrup, was found to be essential, especially for later harvest dates. Despite these interventions, a late harvest (early November) consistently resulted in suboptimal fermentation. The results suggest that optimizing harvest timing and incorporating appropriate additives are key strategies for producing high quality silage and ensuring the suitability of Silphium perfoliatum fibers for industrial applications.
Effects of pretreatment with a ball mill on methane yield of horse manure
(2023) Heller, René; Roth, Peter; Hülsemann, Benedikt; Böttinger, Stefan; Lemmer, Andreas; Oechsner, Hans
Lignocellulosic biomass is an abundant organic material, which can be utilised in biogas plants for sustainable production of biogas. Since these substrates usually have high lignin contents and consist of rather elongated particles, a special pretreatment is required for an economical and process-stable utilisation in the biogas plant. The mechanical pretreatment of horse manure was carried out with the prototype of a ball mill at different speeds. The aim of ball milling is to comminute the substrate and disintegrate the lignocellulosic bond. Mechanical pretreatment in the ball mill resulted in a significant increase in specific methane yield of more than 37% in anaerobic batch digestion (up to 243 LCH4 kgVS−1) of horse manure. The kinetics of the methane gas formation process was analysed by a modified Gompertz model fitting and showed a higher methane production potential and maximum daily methane production rate as well as a lower duration of the lag phase after pretreatment at 6 rpm. This was further confirmed by sieve analyses, which showed a significant reduction of particle size compared to the untreated variant. Thus, the use of the ball mill increases the specific methane yield and improves the fermentation of lignocellulosic substrates such as horse manure.
Fed-batch bioreactor cultivation of Bacillus subtilis using vegetable juice as an alternative carbon source for lipopeptides production: a shift towards a circular bioeconomy
(2024) Gugel, Irene; Vahidinasab, Maliheh; Benatto Perino, Elvio Henrique; Hiller, Eric; Marchetti, Filippo; Costa, Stefania; Pfannstiel, Jens; Konnerth, Philipp; Vertuani, Silvia; Manfredini, Stefano; Hausmann, Rudolf; Gudiña, Eduardo
In a scenario of increasing alarm about food waste due to rapid urbanization, population growth and lifestyle changes, this study aims to explore the valorization of waste from the retail sector as potential substrates for the biotechnological production of biosurfactants. With a perspective of increasingly contributing to the realization of the circular bioeconomy, a vegetable juice, derived from unsold fruits and vegetables, as a carbon source was used to produce lipopeptides such as surfactin and fengycin. The results from the shake flask cultivations revealed that different concentrations of vegetable juice could effectively serve as carbon sources and that the fed-batch bioreactor cultivation strategy allowed the yields of lipopeptides to be significantly increased. In particular, the product/substrate yield of 0.09 g/g for surfactin and 0.85 mg/g for fengycin was obtained with maximum concentrations of 2.77 g/L and 27.53 mg/L after 16 h, respectively. To conclude, this study provides the successful fed-batch cultivation of B. subtilis using waste product as the carbon source to produce secondary metabolites. Therefore, the consumption of agricultural product wastes might be a promising source for producing valuable metabolites which have promising application potential to be used in several fields of biological controls of fungal diseases.
Herstellung von HMF aus Kartoffelschalen
(2025) Limbach, Nadine; Konnerth, Philipp; Kruse, Andrea
Die stoffliche Nutzung von Biomasse zur Herstellung von Plattformchemikalien gewinnt zunehmend an Bedeutung für eine nachhaltigere Chemie. Eine wichtige Verbindung in diesem Bereich ist 5-Hydroxymethylfurfural (HMF), das aus einfachen Zuckern gebildet werden kann. Ziel dieser Arbeit war es, HMF aus dem stärkehaltigen Nebenprodukt der Kartoffelschale zu synthetisieren. Dazu wurden die Einflüsse zweier Mineralsäuren – Schwefelsäure und Salpetersäure – in unterschiedlichen Konzentrationen (1 M, 1,5 M und 2 M) untersucht. Die experimentelle Arbeit bestand aus zwei aufeinanderfolgenden Schritten. Zunächst wurde die Stärke der Kartoffelschalen hydrolytisch aufgeschlossen, um eine möglichst hohe Glucoseausbeute zu erzielen. Im anschließenden Versuch wurde diese Glucose über Isomerisierungs- und Dehydratisierungsschritte zu HMF umgesetzt. Hierfür wurden die Reaktionslösungen auf verschiedene pH-Startwerte (pH 2, pH 2,5 und pH 3) eingestellt. Die Ergebnisse zeigen, dass beide Säuren die Stärkehydrolyse in ähnlicher Weise katalysieren und vergleichbare Ausbeuten an Glucose, Fructose und Zucker-Dimeren bei gleicher Verweilzeit liefern. In der nachfolgenden HMF-Synthese traten jedoch deutliche Unterschiede zwischen den Säuren auf: Schwefelsäure führte zu einer schnelleren Zuckerumwandlung und zu höheren HMF-Ausbeuten bei kürzerer Reaktionszeit. Mit sinkendem pH-Wert stiegen die HMF-Ausbeuten bei beiden Säuren an. Neben HMF entstanden weitere Neben- und Abbauprodukte wie Levulinsäure, Ameisensäure und Huminstoffe. Dabei bildete sich bei Verwendung von Schwefelsäure eine höhere Menge an Huminstoffen als bei Salpetersäure. Insgesamt zeigt sich, dass Schwefelsäure die beteiligten Reaktionen bei gleichem pH-Startwert stärker katalysiert.
How fluid pseudoplasticity and elasticity affect propeller flows in biogas fermenters
(2024) Kolano, Markus; Ohnmacht, Benjamin; Lemmer, Andreas; Kraume, Matthias
Mixing in biogas fermenters is complex due to the non‐Newtonian rheology of biogenic substrates, which exhibit both pseudoplasticity and elasticity. It is yet unclear how these non‐Newtonian properties affect propeller flows and the mixing behavior in fermenters. Therefore, propeller flows in Newtonian as well as shear‐thinning inelastic and elastic fluids are compared numerically and validated against particle image velocity (PIV) data. Elastic normal stresses lead to an increase of pumping rates in the laminar regime and a suppression of the formation of a propeller jet in the transitional regime. Thus, flow rates are severely overestimated by the inelastic, shear‐thinning model in this regime. The results indicate that elasticity is critical for an accurate modeling of flows of biogenic substrates.
Hydrothermal carbonization of fructose—effect of salts and reactor stirring on the growth and formation of carbon spheres
(2021) Jung, Dennis; Duman, Gözde; Zimmermann, Michael; Kruse, Andrea; Yanik, Jale
Hydrothermal carbonization (HTC) has become a promising technology for the production of hydrochar and carbon spheres. Several studies indicate a strong dependency of the reaction conditions on the sphere diameter. The usage of additives, such as salts, is one possibility to increase the size of the spheres. However, the growth mechanism which leads to larger particles is not fully understood. In this work, kinetic studies of HTC with fructose were performed with different salts as additives. The growth of the particles (the increase in size) has been compared to the formation rates (increase in yield) of hydrochar by using the reaction rate constants from the kinetic model. The results indicate that the acceleration of the growth rate is independent of the formation rate. It is therefore assumed that coagulation, as a growth mechanism, took place. With longer reaction times, the particles reached a stable particle size, independently from the added salts; therefore, it was assumed that the particles underwent some sort of solidification. The state of matter can therefore be described as an intermediate state between liquid and solid, similar to mesophase pitch. Experiments with a stirrer resulted in squashed particles, which supports the model, that the particles exhibit emulsion-like behavior.

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