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Article
2025
A diamine oxidase from Glutamicibacter halophytocola for the degradation of histamine and tyramine in foods
A diamine oxidase from Glutamicibacter halophytocola for the degradation of histamine and tyramine in foods
Abstract
A novel diamine oxidase (DAO) was discovered in the bacterium Glutamicibacter halophytocola (DAO-GH). The gene of DAO-GH was integrated into the genome of the yeast Komagataella phaffii and recombinantly produced under control of the methanol-inducible AOX1 promoter in a bioreactor cultivation. A high DAO activity of 70.2 ± 5.2 µkat/Lculture (5.25 ± 0.22 µkat/gprotein) was yielded after 90 h of cultivation. The DAO-GH was partially purified by the polyethyleneimine precipitation of nucleic acids, fractionated ammonium sulfate precipitation and hydrophobic interaction chromatography, resulting in a specific DAO activity of 19.7 µkat/gProtein. The DAO-GH was then biochemically investigated regarding its potential for histamine and tyramine degradation in fermented foods and the human small intestine. Interestingly, the DAO-GH showed activity even at a low pH of 5 and low temperature of 6 °C. Both histamine and tyramine were effectively degraded and DAO-GH showed especially very high affinity towards tyramine (Km of 0.009 mM). The DAO-GH was shown to be capable of degrading around 20% of the initially applied histamine in tuna paste (pH 5.6) at 5 °C within 24 h and completely degraded the histamine in a simulated intestinal fluid within 1.5 h in bioconversion experiments. The DAO-GH was spray-dried for the production of a storable enzyme preparation. Only around 17% of activity were lost in this process and the DAO-GH remained stable at room temperature for at least 3 months. The discovery of this DAO with its very advantageous biochemical properties allows the preparation of histamine-reduced or -free fermented foods by a simple enzymatic treatment or the treatment of histamine intolerance symptoms as a dietary supplement or medicine.
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Foods, 14 (2025), 17, 3093.
https://doi.org/10.3390/foods14173093.
ISSN: 2304-8158
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Kettner, L., Freund, A., Bechtel, A., Costa-Catala, J., & Fischer, L. (2025). A Diamine Oxidase from Glutamicibacter halophytocola for the Degradation of Histamine and Tyramine in Foods. Foods, 14(17). https://doi.org/10.3390/foods14173093
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English
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570 Biology
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Sustainable Development Goals
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@article{Kettner2025,
doi = {10.3390/foods14173093},
author = {Kettner, Lucas and Freund, Alexander and Bechtel, Anna et al.},
title = {A Diamine Oxidase from Glutamicibacter halophytocola for the Degradation of Histamine and Tyramine in Foods},
journal = {Foods},
year = {2025},
volume = {14},
number = {17},
}