A new version of this entry is available:
Loading...
Article
2025
Animal‐free setup of a 3D mature adipocyte‐macrophage co‐culture to induce inflammation in vitro
Animal‐free setup of a 3D mature adipocyte‐macrophage co‐culture to induce inflammation in vitro
Abstract (English)
Adipose tissue inflammation plays a central role in the pathogenesis of metabolic disorders. It is closely associated with immune cell infiltration, particularly macrophages, and the release of pro‐inflammatory cytokines. Reliable in vitro test systems that mimic the inflamed environment while being free of animal‐derived components are essential to explore new treatments for obesity‐related diseases. This study aims to develop a straightforward, animal‐free adipocyte‐macrophage co‐culture for investigating adipose tissue inflammation. Therefore, the human monocytic cell lines Mono Mac (MM6) and THP‐1 are co‐cultured with human primary mature adipocytes (ACs) encapsulated in gellan gum (GG) within a defined environment. Both monocytic cell lines are effectively activated by phorbol 12‐myristate 13‐acetate (PMA) and lipopolysaccharide (LPS) in the defined medium, exhibiting distinct cytokine profiles. A comparison between collagen and GG demonstrates that GG is a suitable animal‐free matrix material for ACs. PMA+LPS successfully activates the 3D adipocyte‐macrophage co‐culture to an inflammatory state for 72 h in the developed defined medium. Viability and intracellular lipid content remain high, and the functionality of ACs (perilipin A) in untreated models remains intact. This inflamed adipocyte‐macrophage co‐culture is easy to assemble and set up in a defined environment, making it a potential test system for anti‐inflammatory treatment strategies.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Other version
Notes
Publication license
Publication series
Published in
Advanced healthcare materials, 14 (2025), 22, 2500779.
https://doi.org/10.1002/adhm.202500779.
ISSN: 2192-2659
ISSN: 2192-2640
Other version
Faculty
Institute
Examination date
Supervisor
Cite this publication
Nowakowski, S., Nellinger, S., Albrecht, F. B., & Kluger, P. J. (2025). Animal‐free setup of a 3D mature adipocyte‐macrophage co‐culture to induce inflammation in vitro. Advanced healthcare materials, 14(22). https://doi.org/10.1002/adhm.202500779
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
610 Medicine and health
Collections
Original object
University bibliography
Standardized keywords (GND)
Sustainable Development Goals
BibTeX
@article{Nowakowski2025,
doi = {10.1002/adhm.202500779},
author = {Nowakowski, Sophia and Nellinger, Svenja and Albrecht, Franziska Brigitte et al.},
title = {Animal‐free setup of a 3D mature adipocyte‐macrophage co‐culture to induce inflammation in vitro},
journal = {Advanced healthcare materials},
year = {2025},
volume = {14},
number = {22},
pages = {--},
}